BACKGROUND: Nanoparticles are being rapidly assimilated into numerous research fields and consumer products. A concurrent increase in human exposure to such materials is expected. Magnetic nanoparticles (MNPs) possess unique and beneficial features, increasing their functionality and integrative potential. However, MNP toxicity characterization is limited, especially in regards to the human respiratory system. This study aimed to assess the in vitro effects of airborne MNPs on BEAS-2B cells. Uncoated iron oxide was compared with two amorphous silica-coated MNPs, hypothesizing the coatings reduced toxicity and increased particle stability. METHOD: BEAS-2B cells were cultured at an air-liquid interface and exposed to airborne MNPs using a fabricated exposure device. Indices of cytotoxicity, inflammatory response, oxidative stress, and iron homeostasis were monitored postexposure via cell viability assays and qRT-PCR. Concentrations of soluble iron-associated with different MNPs were also examined before and after contact with several aqueous organic and inorganic acids. RESULTS: The silica-coated MNPs had reduced soluble iron concentrations. This result indicates that the silica coating provides a barrier to and prevents the mobilization of soluble iron from the particle to the cell, thereby reducing the risk of oxidative stress or alterations of iron homeostasis. Cells exposed to MagSilica50 and MagSilica50-85® showed little to no indications of cytotoxicity or induction of inflammatory response/oxidative stress at the examined delivery concentrations. CONCLUSION: MNPs coated with amorphous silica are protected from acidic erosion. Correspondingly, the particle stability translates into reduced cytotoxicity and cellular influence on human airway epithelial cells.
BACKGROUND: Nanoparticles are being rapidly assimilated into numerous research fields and consumer products. A concurrent increase in human exposure to such materials is expected. Magnetic nanoparticles (MNPs) possess unique and beneficial features, increasing their functionality and integrative potential. However, MNP toxicity characterization is limited, especially in regards to the human respiratory system. This study aimed to assess the in vitro effects of airborne MNPs on BEAS-2B cells. Uncoated iron oxide was compared with two amorphous silica-coated MNPs, hypothesizing the coatings reduced toxicity and increased particle stability. METHOD: BEAS-2B cells were cultured at an air-liquid interface and exposed to airborne MNPs using a fabricated exposure device. Indices of cytotoxicity, inflammatory response, oxidative stress, and iron homeostasis were monitored postexposure via cell viability assays and qRT-PCR. Concentrations of soluble iron-associated with different MNPs were also examined before and after contact with several aqueous organic and inorganic acids. RESULTS: The silica-coated MNPs had reduced soluble iron concentrations. This result indicates that the silica coating provides a barrier to and prevents the mobilization of soluble iron from the particle to the cell, thereby reducing the risk of oxidative stress or alterations of iron homeostasis. Cells exposed to MagSilica50 and MagSilica50-85® showed little to no indications of cytotoxicity or induction of inflammatory response/oxidative stress at the examined delivery concentrations. CONCLUSION: MNPs coated with amorphous silica are protected from acidic erosion. Correspondingly, the particle stability translates into reduced cytotoxicity and cellular influence on human airway epithelial cells.
Authors: Leona D Scanlan; Robert B Reed; Alexandre V Loguinov; Philipp Antczak; Abderrahmane Tagmount; Shaul Aloni; Daniel Thomas Nowinski; Pauline Luong; Christine Tran; Nadeeka Karunaratne; Don Pham; Xin Xin Lin; Francesco Falciani; Christopher P Higgins; James F Ranville; Chris D Vulpe; Benjamin Gilbert Journal: ACS Nano Date: 2013-12-05 Impact factor: 15.881
Authors: Sebastian Oeder; Tamara Kanashova; Olli Sippula; Sean C Sapcariu; Thorsten Streibel; Jose Manuel Arteaga-Salas; Johannes Passig; Marco Dilger; Hanns-Rudolf Paur; Christoph Schlager; Sonja Mülhopt; Silvia Diabaté; Carsten Weiss; Benjamin Stengel; Rom Rabe; Horst Harndorf; Tiina Torvela; Jorma K Jokiniemi; Maija-Riitta Hirvonen; Carsten Schmidt-Weber; Claudia Traidl-Hoffmann; Kelly A BéruBé; Anna J Wlodarczyk; Zoë Prytherch; Bernhard Michalke; Tobias Krebs; André S H Prévôt; Michael Kelbg; Josef Tiggesbäumker; Erwin Karg; Gert Jakobi; Sorana Scholtes; Jürgen Schnelle-Kreis; Jutta Lintelmann; Georg Matuschek; Martin Sklorz; Sophie Klingbeil; Jürgen Orasche; Patrick Richthammer; Laarnie Müller; Michael Elsasser; Ahmed Reda; Thomas Gröger; Benedikt Weggler; Theo Schwemer; Hendryk Czech; Christopher P Rüger; Gülcin Abbaszade; Christian Radischat; Karsten Hiller; Jeroen T M Buters; Gunnar Dittmar; Ralf Zimmermann Journal: PLoS One Date: 2015-06-03 Impact factor: 3.240
Authors: Nagarjun V Konduru; Kimberly M Murdaugh; Georgios A Sotiriou; Thomas C Donaghey; Philip Demokritou; Joseph D Brain; Ramon M Molina Journal: Part Fibre Toxicol Date: 2014-09-03 Impact factor: 9.400