OBJECTIVE: To investigate the immunomodulatory effects of polysaccharides from Panax japonicus on immunosuppressed mice. METHODS: Immunosuppressed murine model were established in Kuming mice induced by cyclophosphamide (Cy) and splenic indexes were evaluated. The proliferation activities of splenic lymphocyte were assayed by MTT and levels of serum hemolysin were confirmed by spectrophotometry. The antibody-secreting capabilities of splenocytes were assayed by quan-titative haemolysis of SRBC (QHS). The percentage of NK cells were analysed by flow cytometry (FCM). RESULTS: Polysaccharides of Panax japonicus significantly increased the splenic indexes, promoted the proliferation of splenic lymphocytes, enhanced QHS reaction, elevated the production of serum hemolysin and percentages of peripheral blood NK cells in Cy-induced immunosuppressed mice. CONCLUSION: Panax japonicus polysaccharides can recover immune function in the Cy-induced immunosuppressed mice.
OBJECTIVE: To investigate the immunomodulatory effects of polysaccharides from Panax japonicus on immunosuppressed mice. METHODS: Immunosuppressed murine model were established in Kuming mice induced by cyclophosphamide (Cy) and splenic indexes were evaluated. The proliferation activities of splenic lymphocyte were assayed by MTT and levels of serum hemolysin were confirmed by spectrophotometry. The antibody-secreting capabilities of splenocytes were assayed by quan-titative haemolysis of SRBC (QHS). The percentage of NK cells were analysed by flow cytometry (FCM). RESULTS:Polysaccharides of Panax japonicus significantly increased the splenic indexes, promoted the proliferation of splenic lymphocytes, enhanced QHS reaction, elevated the production of serum hemolysin and percentages of peripheral blood NK cells in Cy-induced immunosuppressed mice. CONCLUSION:Panax japonicuspolysaccharides can recover immune function in the Cy-induced immunosuppressed mice.
Authors: Zhang Jie; Li Chun-Yan; Li Jing-Ping; Guo Ren; Wang Hui; Pan Juan; Liu Sheng-Lan Journal: Evid Based Complement Alternat Med Date: 2015-08-04 Impact factor: 2.629