| Literature DB >> 21818753 |
Kimberly J Nelson1, Derek Parsonage.
Abstract
Peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. Protocols are provided to measure Prx activity with peroxide by (1) a coupled reaction with NADPH, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the FOX assay.Entities:
Mesh:
Substances:
Year: 2011 PMID: 21818753 PMCID: PMC3703631 DOI: 10.1002/0471140856.tx0710s49
Source DB: PubMed Journal: Curr Protoc Toxicol ISSN: 1934-9254