| Literature DB >> 21815102 |
Takuya Morimoto1, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara.
Abstract
A genetic tool for introducing marker-free deletions is essential for multiple manipulations of genomes. We have developed a simple and efficient method for creating marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool.Entities:
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Year: 2011 PMID： 21815102 DOI： 10.1007/978-1-61779-197-0_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745