Literature DB >> 21815058

Microglia cell culture: a primer for the novice.

Anke Witting1, Thomas Möller.   

Abstract

Microglial cells are the resident immune cells of the central nervous system. Progress in the recent decade has clearly established that microglial cells participate or even actively drive neurological disease. Much of our current knowledge has been generated by investigating microglial cells in cell culture. The aim of this chapter is to give the uninitiated a basic and adaptable protocol for the culturing of microglial cells. We discuss the challenges of microglial cell culture and provide a collection of tips which reflect our 25+ years of collective experience.

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Year:  2011        PMID： 21815058     DOI： 10.1007/978-1-61779-170-3_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures.

Authors:  Christopher J Bohlen; F Chris Bennett; Andrew F Tucker; Hannah Y Collins; Sara B Mulinyawe; Ben A Barres
Journal:  Neuron       Date:  2017-05-17       Impact factor: 17.173

2.  Nerve Injury-Induced Neuronal PAP-I Maintains Neuropathic Pain by Activating Spinal Microglia.

Authors:  Jiayin Li; Haixiang Shi; Hui Liu; Fei Dong; Zhiping Liu; Yingjin Lu; Luonan Chen; Lan Bao; Xu Zhang
Journal:  J Neurosci       Date:  2019-11-19       Impact factor: 6.167

3.  Ischemia/Reperfusion Induces Interferon-Stimulated Gene Expression in Microglia.

Authors:  Ashley McDonough; Richard V Lee; Shahani Noor; Chungeun Lee; Thu Le; Michael Iorga; Jessica L H Phillips; Sean Murphy; Thomas Möller; Jonathan R Weinstein
Journal:  J Neurosci       Date:  2017-07-26       Impact factor: 6.167

4.  Co-Culture of Neurons and Microglia.

Authors:  Pamela J Roqué; Lucio G Costa
Journal:  Curr Protoc Toxicol       Date:  2017-11-08

5.  Microglia mediate diesel exhaust particle-induced cerebellar neuronal toxicity through neuroinflammatory mechanisms.

Authors:  Pamela J Roqué; Khoi Dao; Lucio G Costa
Journal:  Neurotoxicology       Date:  2016-08-16       Impact factor: 4.294

6.  CCL2 Produced by the Glioma Microenvironment Is Essential for the Recruitment of Regulatory T Cells and Myeloid-Derived Suppressor Cells.

Authors:  Alan L Chang; Jason Miska; Derek A Wainwright; Mahua Dey; Claudia V Rivetta; Dou Yu; Deepak Kanojia; Katarzyna C Pituch; Jian Qiao; Peter Pytel; Yu Han; Meijing Wu; Lingjiao Zhang; Craig M Horbinski; Atique U Ahmed; Maciej S Lesniak
Journal:  Cancer Res       Date:  2016-08-16       Impact factor: 12.701

7.  A Subpopulation of Microglia Generated in the Adult Mouse Brain Originates from Prominin-1-Expressing Progenitors.

Authors:  Katherine E Prater; Macarena S Aloi; Jasmine L Pathan; Chloe N Winston; Rachel A Chernoff; Stephanie Davidson; Matthew Sadgrove; Ashley McDonough; Dannielle Zierath; Wei Su; Jonathan R Weinstein; Gwenn A Garden
Journal:  J Neurosci       Date:  2021-08-11       Impact factor: 6.167

8.  Transcriptome sequencing of microglial cells stimulated with TLR3 and TLR4 ligands.

Authors:  Amitabh Das; Jin Choul Chai; Sun Hwa Kim; Young Seek Lee; Kyoung Sun Park; Kyoung Hwa Jung; Young Gyu Chai
Journal:  BMC Genomics       Date:  2015-07-10       Impact factor: 3.969

9.  Dual RNA sequencing reveals the expression of unique transcriptomic signatures in lipopolysaccharide-induced BV-2 microglial cells.

Authors:  Amitabh Das; Jin Choul Chai; Sun Hwa Kim; Kyoung Sun Park; Young Seek Lee; Kyoung Hwa Jung; Young Gyu Chai
Journal:  PLoS One       Date:  2015-03-26       Impact factor: 3.240

10.  Microglial activation mediates host neuronal survival induced by neural stem cells.

Authors:  Hui-Mei Wu; Li-Feng Zhang; Pei-Shang Ding; Ya-Jing Liu; Xu Wu; Jiang-Ning Zhou
Journal:  J Cell Mol Med       Date:  2014-04-13       Impact factor: 5.310
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