Zhangwei Xu1, Hai Shi, Qiao Mei, Yuxian Shen, Jianming Xu. 1. Department of Gastroenterology, the First Affiliated Hospital, Anhui Medical University, Hefei, 230022 Anhui Province, People's Republic of China.
Abstract
BACKGROUND AND AIM: Previous studies have shown that overexpression of macrophage metalloelastase (MME) suppresses tumor growth in mice. The purpose of this study was to investigate the effects of MME on basic fibroblast growth factor (bFGF) expression and tumor angiogenesis in murine colon cancer. METHODS: Murine CT-26 colon cancer cells stably transfected with MME were inoculated subcutaneously. Reverse-transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry were used to explore the bFGF mRNA and protein expression. Immunohistochemical staining of CD34 was used to measure the microvessel density (MVD). RESULTS: bFGF mRNA levels in tumor tissues of CT-26-EGFP and nontransfected cells were respectively 2.7-fold (0.56 ± 0.063 vs. 0.21 ± 0.042) and 2.5-fold (0.53 ± 0.066 vs. 0.21 ± 0.042) higher than that in tumors of CT-26-EGFP-MME cells (p < 0.01). bFGF protein levels exhibited a similar trend. Tumors of CT-26-EGFP-MME cells demonstrated a lower microvessel density (9.35 ± 2.79) than control tumors of CT-26-EGFP cells (22.85 ± 3.80) and nontransfected cells (23.45 ± 4.49) (p < 0.001). CONCLUSIONS: We found that expression of MME inversely correlates with the expression of bFGF and tumor angiogenesis in a model of murine colon cancer. These data indicate that manipulation of MME expression could be a novel modality approach to colon cancer therapy.
BACKGROUND AND AIM: Previous studies have shown that overexpression of macrophage metalloelastase (MME) suppresses tumor growth in mice. The purpose of this study was to investigate the effects of MME on basic fibroblast growth factor (bFGF) expression and tumor angiogenesis in murinecolon cancer. METHODS:Murine CT-26 colon cancer cells stably transfected with MME were inoculated subcutaneously. Reverse-transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry were used to explore the bFGF mRNA and protein expression. Immunohistochemical staining of CD34 was used to measure the microvessel density (MVD). RESULTS:bFGF mRNA levels in tumor tissues of CT-26-EGFP and nontransfected cells were respectively 2.7-fold (0.56 ± 0.063 vs. 0.21 ± 0.042) and 2.5-fold (0.53 ± 0.066 vs. 0.21 ± 0.042) higher than that in tumors of CT-26-EGFP-MME cells (p < 0.01). bFGF protein levels exhibited a similar trend. Tumors of CT-26-EGFP-MME cells demonstrated a lower microvessel density (9.35 ± 2.79) than control tumors of CT-26-EGFP cells (22.85 ± 3.80) and nontransfected cells (23.45 ± 4.49) (p < 0.001). CONCLUSIONS: We found that expression of MME inversely correlates with the expression of bFGF and tumor angiogenesis in a model of murinecolon cancer. These data indicate that manipulation of MME expression could be a novel modality approach to colon cancer therapy.
Authors: I Vlodavsky; Z Fuks; R Ishai-Michaeli; P Bashkin; E Levi; G Korner; R Bar-Shavit; M Klagsbrun Journal: J Cell Biochem Date: 1991-02 Impact factor: 4.429
Authors: Yves A DeClerck; Arthur M Mercurio; M Sharon Stack; Harold A Chapman; Mary M Zutter; Ruth J Muschel; Avraham Raz; Lynn M Matrisian; Bonnie F Sloane; Agnes Noel; Mary J Hendrix; Lisa Coussens; Martin Padarathsingh Journal: Am J Pathol Date: 2004-04 Impact factor: 4.307