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Literature DB >> 2181402

Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus.

Abstract

Sequences encoding adenovirus type 2 DNA polymerase were placed under control of the polyhedrin promoter and inserted into the baculovirus Autographa californica nuclear polyhedrosis virus by homologous recombination. Insect cells infected with the recombinant virus produced substantial amounts of the adenovirus type 2 DNA polymerase protein which was functional in both DNA polymerase and replication initiation reactions. Thus, the baculovirus expression system can provide active adenovirus type 2 DNA polymerase that is produced in quantities suitable for biochemical and structural analysis.

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Year: 1990 PMID： 2181402 PMCID： PMC330431 DOI： 10.1093/nar/18.5.1167

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

23 in total

6. Influence of codon usage and translational initiation codon context in the AcNPV-based expression system: computer analysis using homologous and heterologous genes.

Authors: A Ranjan; S E Hasnain
Journal: Virus Genes Date: 1995-01 Impact factor: 2.332

7. Recognition of the adenovirus type 2 origin of DNA replication by the virally encoded DNA polymerase and preterminal proteins.

Authors: S M Temperley; R T Hay
Journal: EMBO J Date: 1992-02 Impact factor: 11.598

7 in total

Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus.

1. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.

2. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

3. Baculovirus induction of a DNA polymerase.

4. The structure of replicating adenovirus 2 DNA molecules.

5. Structure and organization of the gene coding for the DNA binding protein of adenovirus type 5.

6. Strong and regulated expression of Escherichia coli beta-galactosidase in insect cells with a baculovirus vector.

7. The infectivity of adenovirus genomes lacking DNA sequences from their left-hand termini.

8. Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA.

9. Formation of a covalent complex between the 80,000-dalton adenovirus terminal protein and 5'-dCMP in vitro.

10. Adenovirus DNA replication in vitro: synthesis of full-length DNA with purified proteins.

1. Adenovirus DNA polymerase: domain organisation and interaction with preterminal protein.

2. Role of preterminal protein processing in adenovirus replication.

3. Activation of adenovirus-coded protease and processing of preterminal protein.

4. Mutations in two cysteine-histidine-rich clusters in adenovirus type 2 DNA polymerase affect DNA binding.

5. Targeting of DNA polymerase to the adenovirus origin of DNA replication by interaction with nuclear factor I.

6. Influence of codon usage and translational initiation codon context in the AcNPV-based expression system: computer analysis using homologous and heterologous genes.

7. Recognition of the adenovirus type 2 origin of DNA replication by the virally encoded DNA polymerase and preterminal proteins.