Literature DB >> 21813723

Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation.

M Kramski1, A J Gaeguta, G F Lichtfuss, R Rajasuriar, S M Crowe, M A French, S R Lewin, R J Center, D F J Purcell.   

Abstract

We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.

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Year:  2011        PMID： 21813723      PMCID： PMC3187295          DOI： 10.1128/JCM.01018-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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