AIM: With regards to nanoparticles, all biomedical applications require cellular uptake, which to date remains a hurdle to further progress. This study aims to compare both the attractive force of a static magnetic field and the cell penetrating capability of penetratin; two techniques currently employed to enhance cell uptake. MATERIALS & METHODS: Fluorescent magnetic nanoparticles were functionalized with penetratin and cells were challenged with or without the particles in the presence/absence of a static magnetic field (350 mT). Following analysis of the magnetic field applied, cellular uptake and behavior was assessed in terms of fluorescence microscopy, clathrin and caveolin levels, scanning electron microscopy and transmission electron microscopy. RESULTS: Modeling of the field applied demonstrated varying field patterns across the cell culture area, reflected by higher particle uptake at higher field strengths. Both penetratin and the magnetic field increased cell uptake with penetratin proving more efficient. Interestingly, the magnetic field stimulated clathrin-mediated endocytosis and subsequent particle uptake.
AIM: With regards to nanoparticles, all biomedical applications require cellular uptake, which to date remains a hurdle to further progress. This study aims to compare both the attractive force of a static magnetic field and the cell penetrating capability of penetratin; two techniques currently employed to enhance cell uptake. MATERIALS & METHODS: Fluorescent magnetic nanoparticles were functionalized with penetratin and cells were challenged with or without the particles in the presence/absence of a static magnetic field (350 mT). Following analysis of the magnetic field applied, cellular uptake and behavior was assessed in terms of fluorescence microscopy, clathrin and caveolin levels, scanning electron microscopy and transmission electron microscopy. RESULTS: Modeling of the field applied demonstrated varying field patterns across the cell culture area, reflected by higher particle uptake at higher field strengths. Both penetratin and the magnetic field increased cell uptake with penetratin proving more efficient. Interestingly, the magnetic field stimulated clathrin-mediated endocytosis and subsequent particle uptake.
Authors: Bartosz F Grześkowiak; Yolanda Sánchez-Antequera; Edelburga Hammerschmid; Markus Döblinger; Dietmar Eberbeck; Anna Woźniak; Ryszard Słomski; Christian Plank; Olga Mykhaylyk Journal: Pharm Res Date: 2014-07-18 Impact factor: 4.200
Authors: Mariana P Nucci; Javier B Mamani; Fernando A Oliveira; Igor S Filgueiras; Arielly H Alves; Matheus H Theinel; Luiz D Rodrigues; Luciana Marti; Lionel F Gamarra Journal: Pharmaceutics Date: 2022-06-12 Impact factor: 6.525
Authors: Natasha S Lewis; Emily El Lewis; Margaret Mullin; Helen Wheadon; Matthew J Dalby; Catherine C Berry Journal: J Tissue Eng Date: 2017-04-24 Impact factor: 7.813
Authors: Suman Chaudhary; Carol Anne Smith; Pablo Del Pino; Jesus M de la Fuente; Margaret Mullin; Andrew Hursthouse; David Stirling; Catherine C Berry Journal: Pharmaceuticals (Basel) Date: 2013-02-06