Literature DB >> 2180951

Photoaffinity labeling of the Klenow fragment with 8-azido-dATP.

J Rush1, W H Konigsberg.   

Abstract

The photoaffinity compound 8-azido-dATP was used as a probe for the deoxyribonucleoside triphosphate-binding site of the large fragment of DNA polymerase I. Azido-dATP specifically modified a saturable binding site within the Klenow fragment, and each of the four natural deoxyribonucleoside triphosphate substrates competed with labeling at this site in proportion to its binding constant, as previously defined by equilibrium dialysis. Analysis of tryptic peptides after azido-dATP modification revealed five major cross-linking products, which apparently arose from five distinct photoadducts formed near Tyr-766.

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Year:  1990        PMID： 2180951

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  8 in total

1.  Mapping of ATP binding regions in poly(A) polymerases by photoaffinity labeling and by mutational analysis identifies a domain conserved in many nucleotidyltransferases.

Authors:  G Martin; P Jenö; W Keller
Journal:  Protein Sci       Date:  1999-11       Impact factor: 6.725

2.  Structural and functional analysis of temperature-sensitive mutants of the phage phi 29 DNA polymerase.

Authors:  M A Blasco; L Blanco; E Parés; M Salas; A Bernad
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

3.  UTP-bound and Apo structures of a minimal RNA uridylyltransferase.

Authors:  Jason Stagno; Inna Aphasizheva; Anja Rosengarth; Hartmut Luecke; Ruslan Aphasizhev
Journal:  J Mol Biol       Date:  2006-12-02       Impact factor: 5.469

4.  Identification of a common protease-sensitive region in D-alanyl-D-alanine and D-alanyl-D-lactate ligases and photoaffinity labeling with 8-azido ATP.

Authors:  G D Wright; C T Walsh
Journal:  Protein Sci       Date:  1993-10       Impact factor: 6.725

5.  Sulphydryl groups in the template-primer-binding domain of murine leukaemia virus reverse transcriptase. Identification and functional analysis of cysteine-90.

Authors:  S Basu; A Basu; M J Modak
Journal:  Biochem J       Date:  1993-12-15       Impact factor: 3.857

6.  Genetic characterization of the vaccinia virus DNA polymerase: cytosine arabinoside resistance requires a variable lesion conferring phosphonoacetate resistance in conjunction with an invariant mutation localized to the 3'-5' exonuclease domain.

Authors:  J A Taddie; P Traktman
Journal:  J Virol       Date:  1993-07       Impact factor: 5.103

7.  Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

Authors:  M Suzuki; D Baskin; L Hood; L A Loeb
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-03       Impact factor: 11.205

8.  Mutations in T7 RNA polymerase that support the proposal for a common polymerase active site structure.

Authors:  G Bonner; D Patra; E M Lafer; R Sousa
Journal:  EMBO J       Date:  1992-10       Impact factor: 11.598
  8 in total

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