Literature DB >> 2180938

Crystallization of recombinant rat cathepsin B.

X Lee1, F R Ahmed, T Hirama, C P Huber, D R Rose, R To, S Hasnain, A Tam, J S Mort.   

Abstract

A glycosylation-minus mutant of rat cathepsin B expressed in yeast has been purified and crystallized. X-ray diffraction data have been collected and molecular replacement for solving the structure is in progress. The space group for the recombinant rat cathepsin B was determined to be P2(1) with unit cell dimensions alpha = 62.2 A, b = 90.19 A, c = 47.07 A, and beta = 97.43 degrees. A unit cell contains 4 molecules and 2 molecules per asymmetric unit.

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Year:  1990        PMID: 2180938

Source DB:  PubMed          Journal:  J Biol Chem        ISSN: 0021-9258            Impact factor:   5.157


  6 in total

1.  Application of protein engineering to enhance crystallizability and improve crystal properties.

Authors:  Zygmunt S Derewenda
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-04-21

2.  Demonstration by electrospray mass spectrometry that the peptidyldipeptidase activity of cathepsin B is capable of rat cathepsin B C-terminal processing.

Authors:  A D Rowan; R Feng; Y Konishi; J S Mort
Journal:  Biochem J       Date:  1993-09-15       Impact factor: 3.857

3.  Heterologous expression and characterization of Schizosaccharomyces pombe vacuolar carboxypeptidase Y in Saccharomyces cerevisiae.

Authors:  Kaoru Takegawa; Sanae Tokudomi; M Shah Alam Bhuiyan; Mitsuaki Tabuchi; Yasuko Fujita; Tomoko Iwaki; Shigeru Utsumi; Naotaka Tanaka
Journal:  Curr Genet       Date:  2002-12-14       Impact factor: 3.886

4.  An approach to crystallizing proteins by metal-mediated synthetic symmetrization.

Authors:  Arthur Laganowsky; Minglei Zhao; Angela B Soriaga; Michael R Sawaya; Duilio Cascio; Todd O Yeates
Journal:  Protein Sci       Date:  2011-09-30       Impact factor: 6.725

5.  Development of a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments: detection of fragments in human synovial fluids.

Authors:  A J Fosang; K Last; P Gardiner; D C Jackson; L Brown
Journal:  Biochem J       Date:  1995-08-15       Impact factor: 3.857

6.  The refined 2.15 A X-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.

Authors:  D Musil; D Zucic; D Turk; R A Engh; I Mayr; R Huber; T Popovic; V Turk; T Towatari; N Katunuma
Journal:  EMBO J       Date:  1991-09       Impact factor: 11.598

  6 in total

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