BACKGROUND: protein glycosylation varies with the physiological and pathological status of the cell. Consequently, analysis of protein-linked glycans has growing importance both in basic glycobiological research and as a potential tool for monitoring the physiological state in humans. DESIGN, SETTING AND PARTICIPANTS: a total of 265 healthy northern Chinese men and women were grouped by age and gender. The mean age in males and females was similar. OBJECTIVE: the study is aimed to evaluate the effects of the age and gender on the human serum N-glycans profiles in the clinical diagnose of ageing and disease. METHODS: the 265 human serum N-glycan profiles were obtained by DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. Comparison of N-glycan profiles was carried out among the different genders and age groups and the data were analysed with the GeneMapper software. RESULTS: age-related changes in the three N-glycan structures (NGA2F, NGA2FB and NA2F) were observed. Interestingly, fucosylation of N-glycans was significantly different (P < 0.0001) between men and women: more core-α-1,6-fucosylated glycans were detected in women, whereas more branching-α-1,3-fucosylated N-glycans were seen in men. CONCLUSIONS: the N-glycome profile in serum is gender and age dependent. This should be taken into consideration in the development of serum glycome markers.
BACKGROUND: protein glycosylation varies with the physiological and pathological status of the cell. Consequently, analysis of protein-linked glycans has growing importance both in basic glycobiological research and as a potential tool for monitoring the physiological state in humans. DESIGN, SETTING AND PARTICIPANTS: a total of 265 healthy northern Chinese men and women were grouped by age and gender. The mean age in males and females was similar. OBJECTIVE: the study is aimed to evaluate the effects of the age and gender on the human serum N-glycans profiles in the clinical diagnose of ageing and disease. METHODS: the 265 human serum N-glycan profiles were obtained by DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. Comparison of N-glycan profiles was carried out among the different genders and age groups and the data were analysed with the GeneMapper software. RESULTS: age-related changes in the three N-glycan structures (NGA2F, NGA2FB and NA2F) were observed. Interestingly, fucosylation of N-glycans was significantly different (P < 0.0001) between men and women: more core-α-1,6-fucosylated glycans were detected in women, whereas more branching-α-1,3-fucosylated N-glycans were seen in men. CONCLUSIONS: the N-glycome profile in serum is gender and age dependent. This should be taken into consideration in the development of serum glycome markers.
Authors: Kamil C Kural; Neetu Tandon; Mikhail Skoblov; Olga V Kel-Margoulis; Ancha V Baranova Journal: BMC Genomics Date: 2016-12-28 Impact factor: 3.969
Authors: Karli R Reiding; L Renee Ruhaak; Hae-Won Uh; Said El Bouhaddani; Erik B van den Akker; Rosina Plomp; Liam A McDonnell; Jeanine J Houwing-Duistermaat; P Eline Slagboom; Marian Beekman; Manfred Wuhrer Journal: Mol Cell Proteomics Date: 2016-12-08 Impact factor: 5.911
Authors: Altan Ercan; Wendy M Kohrt; Jing Cui; Kevin D Deane; Marija Pezer; Elaine W Yu; Jonathan S Hausmann; Harry Campbell; Ursula B Kaiser; Pauline M Rudd; Gordan Lauc; James F Wilson; Joel S Finkelstein; Peter A Nigrovic Journal: JCI Insight Date: 2017-02-23
Authors: Haiying Li; Viral Patel; Shannon E DiMartino; John W Froehlich; Richard S Lee Journal: Mol Cell Proteomics Date: 2020-07-31 Impact factor: 5.911