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Literature DB >> 21803919

Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.

Sam Abraham¹, James Chin, Huub J M Brouwers, Bernadette Turner, Ren Zhang, Toni A Chapman.

Abstract

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

Entities: Gene

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Year: 2011 PMID： 21803919 PMCID： PMC3187172 DOI： 10.1128/AEM.00534-11

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

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Review 7. The SOS chromotest: a review.

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1 in total