Literature DB >> 21803918

Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes.

Peter C H Feng1, Karen Jinneman, Flemming Scheutz, Steven R Monday.   

Abstract

Specificity analysis for stx or Stx subtypes in Escherichia coli showed that the PCR assays we tested did not detect stx(1d) and stx(2f), and some also missed stx(2b) and stx(2g). Most of the serological assays examined did not detect Stx2c, Stx2e, Stx2f, and Stx2g, and some strain-to-strain variation in reactivity was observed for Stx2b.

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Year:  2011        PMID： 21803918      PMCID： PMC3187163          DOI： 10.1128/AEM.00370-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  22 in total

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2.  Role of the laboratory in the diagnosis of enterohemorrhagic Escherichia coli infections.

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5.  Rapid detection and isolation of shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay.

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7.  A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons.

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8.  Shiga toxin 1c-producing Escherichia coli strains: phenotypic and genetic characterization and association with human disease.

Authors:  Alexander W Friedrich; Julia Borell; Martina Bielaszewska; Angelika Fruth; Helmut Tschäpe; Helge Karch
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  26 in total

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Authors:  Azucena Mora; Cecilia López; Ghizlane Dhabi; Ana M López-Beceiro; Luís E Fidalgo; Eduardo A Díaz; Carlos Martínez-Carrasco; Rosalía Mamani; Alexandra Herrera; Jesús E Blanco; Miguel Blanco; Jorge Blanco
Journal:  Appl Environ Microbiol       Date:  2012-02-03       Impact factor: 4.792

2.  Prevalences of Shiga toxin subtypes and selected other virulence factors among Shiga-toxigenic Escherichia coli strains isolated from fresh produce.

Authors:  Peter C H Feng; Shanker Reddy
Journal:  Appl Environ Microbiol       Date:  2013-08-30       Impact factor: 4.792

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4.  Evaluation of a loop-mediated isothermal amplification suite for the rapid, reliable, and robust detection of Shiga toxin-producing Escherichia coli in produce.

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Review 5.  Shiga toxin-producing Escherichia coli in swine: the public health perspective.

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Journal:  Appl Environ Microbiol       Date:  2014-02-28       Impact factor: 4.792

8.  Evaluation of CHROMagar STEC and STEC O104 chromogenic agar media for detection of Shiga Toxin-producing Escherichia coli in stool specimens.

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Journal:  J Clin Microbiol       Date:  2013-01-02       Impact factor: 5.948

9.  Single-Cell-Based Digital PCR Detection and Association of Shiga Toxin-Producing Escherichia coli Serogroups and Major Virulence Genes.

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10.  A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli.

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