Literature DB >> 21803888

Recombinant protein production by in vivo polymer inclusion display.

Katrin Grage1, Verena Peters, Bernd H A Rehm.   

Abstract

A novel approach to produce purified recombinant proteins was established. The target protein is produced as polyhydroxyalkanoate (PHA) synthase fusion protein, which mediates intracellular formation of PHA inclusions displaying the target protein. After isolation of the PHA inclusions, the pure target protein was released by simple enterokinase digestion.

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Year:  2011        PMID： 21803888      PMCID： PMC3187177          DOI： 10.1128/AEM.05953-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  24 in total

Review 1.  Recombinant antibodies: engineering and production in yeast and bacterial hosts.

Authors:  Ki Jun Jeong; Seung Hoon Jang; Natarajan Velmurugan
Journal:  Biotechnol J       Date:  2011-01       Impact factor: 4.677

2.  In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase.

Authors:  Verena Peters; Bernd H A Rehm
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792

Review 3.  Bioseparation in antibody manufacturing: the good, the bad and the ugly.

Authors:  Uwe Gottschalk
Journal:  Biotechnol Prog       Date:  2008-04-29

4.  Genetic analysis of mutants producing defective beta-galactosidase which can be activated by specific antibodie.

Authors:  F Melchers
Journal:  Mol Gen Genet       Date:  1970

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 6.  Bacterial polyhydroxyalkanoate granules: biogenesis, structure, and potential use as nano-/micro-beads in biotechnological and biomedical applications.

Authors:  Katrin Grage; Anika C Jahns; Natalie Parlane; Rajasekaran Palanisamy; Indira A Rasiah; Jane A Atwood; Bernd H A Rehm
Journal:  Biomacromolecules       Date:  2009-04-13       Impact factor: 6.988

7.  Multifunctional inorganic-binding beads self-assembled inside engineered bacteria.

Authors:  Anika C Jahns; Richard G Haverkamp; Bernd H A Rehm
Journal:  Bioconjug Chem       Date:  2008-09-09       Impact factor: 4.774

8.  In vivo production of scFv-displaying biopolymer beads using a self-assembly-promoting fusion partner.

Authors:  Katrin Grage; Bernd H A Rehm
Journal:  Bioconjug Chem       Date:  2007-12-19       Impact factor: 4.774

9.  The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi.

Authors:  Vivi Joosten; Christien Lokman; Cees AMJJ Van Den Hondel; Peter J Punt
Journal:  Microb Cell Fact       Date:  2003-01-30       Impact factor: 5.328

10.  Microbial factories for recombinant pharmaceuticals.

Authors:  Neus Ferrer-Miralles; Joan Domingo-Espín; José Luis Corchero; Esther Vázquez; Antonio Villaverde
Journal:  Microb Cell Fact       Date:  2009-03-24       Impact factor: 5.328
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  8 in total

1.  Engineering bacteria to manufacture functionalized polyester beads.

Authors:  Jenny L Draper; Bernd H Rehm
Journal:  Bioengineered       Date:  2012-06-18       Impact factor: 3.269

2.  New skin test for detection of bovine tuberculosis on the basis of antigen-displaying polyester inclusions produced by recombinant Escherichia coli.

Authors:  Shuxiong Chen; Natalie A Parlane; Jason Lee; D Neil Wedlock; Bryce M Buddle; Bernd H A Rehm
Journal:  Appl Environ Microbiol       Date:  2014-02-14       Impact factor: 4.792

3.  Engineering Bacillus megaterium for production of functional intracellular materials.

Authors:  Katrin Grage; Paul McDermott; Bernd H A Rehm
Journal:  Microb Cell Fact       Date:  2017-11-22       Impact factor: 5.328

4.  Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions.

Authors:  Jinping Du; Bernd H A Rehm
Journal:  Microb Cell Fact       Date:  2017-11-02       Impact factor: 5.328

5.  In vivo polyester immobilized sortase for tagless protein purification.

Authors:  Iain D Hay; Jinping Du; Patricia Rubio Reyes; Bernd H A Rehm
Journal:  Microb Cell Fact       Date:  2015-11-25       Impact factor: 5.328

6.  Bioengineered polyester beads co-displaying protein and carbohydrate-based antigens induce protective immunity against bacterial infection.

Authors:  Majela González-Miró; Laura M Rodríguez-Noda; Mildrey Fariñas-Medina; Barbara Cedré-Marrero; Sandra Madariaga-Zarza; Caridad Zayas-Vignier; Mabel Hernández-Cedeño; Torsten Kleffmann; Dagmar García-Rivera; Vicente Vérez-Bencomo; Bernd H A Rehm
Journal:  Sci Rep       Date:  2018-01-30       Impact factor: 4.379

7.  Self-assembled particulate PsaA as vaccine against Streptococcus pneumoniae infection.

Authors:  Majela González-Miro; Laura Rodríguez-Noda; Mildrey Fariñas-Medina; Dagmar García-Rivera; Vicente Vérez-Bencomo; Bernd H A Rehm
Journal:  Heliyon       Date:  2017-04-11

Review 8.  Smart polyhydroxyalkanoate nanobeads by protein based functionalization.

Authors:  Nina Dinjaski; M Auxiliadora Prieto
Journal:  Nanomedicine       Date:  2015-02-24       Impact factor: 5.307
  8 in total

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