| Literature DB >> 21803020 |
Jordan D Dimitrov1, Sebastien Lacroix-Desmazes, Srinivas V Kaveri.
Abstract
The evaluation of antibody avidity by elution with chaotropic agents is a frequently used approach in research and diagnostics. It provides important information on the functional relevance of antibodies. However, in the literature, there is a large heterogeneity in the experimental settings for the determination of this important parameter. Here, we demonstrate that antibody concentration and the choice of chaotropic agent are critical for the reliable estimation of antibody avidity.Mesh：
Substances：
Year: 2011 PMID： 21803020 DOI： 10.1016/j.ab.2011.07.007
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365