| Literature DB >> 21798894 |
Davide Corti1, Jarrod Voss, Steven J Gamblin, Giosiana Codoni, Annalisa Macagno, David Jarrossay, Sebastien G Vachieri, Debora Pinna, Andrea Minola, Fabrizia Vanzetta, Chiara Silacci, Blanca M Fernandez-Rodriguez, Gloria Agatic, Siro Bianchi, Isabella Giacchetto-Sasselli, Lesley Calder, Federica Sallusto, Patrick Collins, Lesley F Haire, Nigel Temperton, Johannes P M Langedijk, John J Skehel, Antonio Lanzavecchia.
Abstract
The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.Entities:
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Year: 2011 PMID： 21798894 DOI： 10.1126/science.1205669
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728