Literature DB >> 21796520

Ligand competition binding assay for the androgen receptor.

Clémentine Féau1, Leggy A Arnold, Aaron Kosinski, R Kiplin Guy.   

Abstract

Evaluating endocrine activities of environmental chemicals or screening for new small molecule modulators of the androgen receptor (AR) transcription activity requires standardized and reliable assay procedures. Scintillation proximity assays (SPA) are sensitive and reliable techniques that are suitable for ligand competition binding assays. We have utilized a radiolabeled ligand competition binding assay for the androgen receptor (AR) that can be carried out in a 384-well format. This standardized, highly reproducible and low-cost assay has been automated for high-throughput screening (HTS) purposes.

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Year:  2011        PMID： 21796520     DOI： 10.1007/978-1-61779-243-4_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

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Journal:  Cancer Epidemiol Biomarkers Prev       Date:  2020-03-09       Impact factor: 4.254

3.  Effect of small molecules modulating androgen receptor (SARMs) in human prostate cancer models.

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Journal:  PLoS One       Date:  2013-05-08       Impact factor: 3.240
  3 in total

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