PURPOSE: To improve gene transducibility mediated by adenovirus (Ad) in cancer cells and further enhance anti-tumor effects by co-delivery. METHODS: Calcium-induced phase change method was used to prepare the complex of anionic liposomes and adenovirus (AL/Ad5). Gene expression was qualitatively detected by X-gal staining and quantitatively detected by ELISA. Taking adenovirus-mediated stromal cell-derived factor-1α (Ad5-SDF1α) as therapeutic gene and carmustine (BCNU) as chemotherapeutic agent, a co-delivering system of AL/Ad5-SDF1α/BCNU was prepared and administered to tumor-bearing mice by intratumor injection. RESULTS: Enhanced LacZ gene transduction was obtained in B16 and Lewis lung carcinoma cells in vitro and in vivo. Complexes of AL/Ad5-SDF1α improved SDF1α gene expression and led to accumulation of dendritic cells among the murine B16 melanoma cells in vivo. This co-delivery system of AL/Ad5-SDF1α/BCNU could significantly suppress tumor growth and prolong survival of tumor-bearing mice. CONCLUSIONS: Through the co-delivering system, AL/Ad5-SDF1α could synergize with BCNU to improve the antitumor effect. It may be a promising strategy for solid tumor therapy.
PURPOSE: To improve gene transducibility mediated by adenovirus (Ad) in cancer cells and further enhance anti-tumor effects by co-delivery. METHODS:Calcium-induced phase change method was used to prepare the complex of anionic liposomes and adenovirus (AL/Ad5). Gene expression was qualitatively detected by X-gal staining and quantitatively detected by ELISA. Taking adenovirus-mediated stromal cell-derived factor-1α (Ad5-SDF1α) as therapeutic gene and carmustine (BCNU) as chemotherapeutic agent, a co-delivering system of AL/Ad5-SDF1α/BCNU was prepared and administered to tumor-bearing mice by intratumor injection. RESULTS: Enhanced LacZ gene transduction was obtained in B16 and Lewis lung carcinoma cells in vitro and in vivo. Complexes of AL/Ad5-SDF1α improved SDF1α gene expression and led to accumulation of dendritic cells among the murine B16 melanoma cells in vivo. This co-delivery system of AL/Ad5-SDF1α/BCNU could significantly suppress tumor growth and prolong survival of tumor-bearing mice. CONCLUSIONS: Through the co-delivering system, AL/Ad5-SDF1α could synergize with BCNU to improve the antitumor effect. It may be a promising strategy for solid tumor therapy.
Authors: Jason C Steel; Heather M A Cavanagh; Mark A Burton; Mones S Abu-Asab; Maria Tsokos; John C Morris; Wouter H J Kalle Journal: Eur J Pharm Sci Date: 2007-01-08 Impact factor: 4.384
Authors: Jin Kim; Theodore Smith; Neeraja Idamakanti; Kathy Mulgrew; Michele Kaloss; Helen Kylefjord; Patricia C Ryan; Michael Kaleko; Susan C Stevenson Journal: J Virol Date: 2002-02 Impact factor: 5.103
Authors: Christine J Weydert; Yuping Zhang; Wenqing Sun; Trent A Waugh; Melissa L T Teoh; Kelly K Andringa; Nukhet Aykin-Burns; Douglas R Spitz; Brian J Smith; Larry W Oberley Journal: Free Radic Biol Med Date: 2007-11-28 Impact factor: 7.376