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Literature DB >> 21785758

Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe.

Jiangyan Zhang¹, Zhengping Li, Hui Wang, Yucong Wang, Hongxia Jia, Jingli Yan.

Abstract

It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity. This journal is © The Royal Society of Chemistry 2011

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Year: 2011 PMID： 21785758 DOI： 10.1039/c1cc13466c

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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Cited

6 in total

1. Light Scattering Technology-Combined Ligation-Dependent Loop-Mediated Isothermal Amplification (LL-LAMP) for Sensitive Detection of RNA.

Authors: Honghong Wang; Shuhui Wang; Hui Wang; Yuanwen Liang; Yuting Jia; Zhengping Li
Journal: ACS Omega Date: 2022-06-01

2. Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification.

Authors: Honghong Wang; Hui Wang; Xinrui Duan; Yuanyuan Sun; Xiangdong Wang; Zhengping Li
Journal: Chem Sci Date: 2017-03-01 Impact factor: 9.825

3. Identification of a selective DNA ligase for accurate recognition and ultrasensitive quantification of N⁶-methyladenosine in RNA at one-nucleotide resolution.

Authors: Weiliang Liu; Jingli Yan; Zhenhao Zhang; Hongru Pian; Chenghui Liu; Zhengping Li
Journal: Chem Sci Date: 2018-02-15 Impact factor: 9.825

Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe.

1. Light Scattering Technology-Combined Ligation-Dependent Loop-Mediated Isothermal Amplification (LL-LAMP) for Sensitive Detection of RNA.

2. Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification.

3. Identification of a selective DNA ligase for accurate recognition and ultrasensitive quantification of N⁶-methyladenosine in RNA at one-nucleotide resolution.

Review 4. Methodological Approaches to Study Extracellular Vesicle miRNAs in Epstein⁻Barr Virus-Associated Cancers.

5. Establishment and Application of Ligation Reaction-Based Method for Quantifying MicroR-156b.

6. A label-free aptamer-based biosensor for microRNA detection by the RNA-regulated fluorescence of malachite green.

Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe.

1. Light Scattering Technology-Combined Ligation-Dependent Loop-Mediated Isothermal Amplification (LL-LAMP) for Sensitive Detection of RNA.

2. Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification.

3. Identification of a selective DNA ligase for accurate recognition and ultrasensitive quantification of N6-methyladenosine in RNA at one-nucleotide resolution.

Review 4. Methodological Approaches to Study Extracellular Vesicle miRNAs in Epstein⁻Barr Virus-Associated Cancers.

5. Establishment and Application of Ligation Reaction-Based Method for Quantifying MicroR-156b.

6. A label-free aptamer-based biosensor for microRNA detection by the RNA-regulated fluorescence of malachite green.

3. Identification of a selective DNA ligase for accurate recognition and ultrasensitive quantification of N⁶-methyladenosine in RNA at one-nucleotide resolution.