Literature DB >> 21785530

Dissociation constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes.

Chun-Wei Wang1, Rakesh Kapoor.   

Abstract

The document reports a novel method of measuring dissociation constant (k(D)) of antibody-antigen interaction using evanescent wave based combination tapered fiber-optic biosensor (CTFOB) dip-probes. The method was demonstrated by measuring the dissociation constant of human Interleukin-6 (IL-6) and anti-IL-6 interaction. Sandwich immunoassay was used to generate fluorescence signal proportional to antigen-antibody conjugate pairs. A portable CCD based spectrometer setup was used to record spectral profile of the fluorescence signal. The measured value of dissociation constant k(D) for IL-6 and capture anti-IL-6 (clone MQ2-13A5) antibodies at room temperature is588 ±19 pM .

Entities:  

Year:  2010        PMID： 21785530      PMCID： PMC3140283          DOI： 10.1117/12.843720

Source DB:  PubMed          Journal:  Proc SPIE Int Soc Opt Eng        ISSN： 0277-786X


  6 in total

1.  Measurement of association rate constant of antibody-antigen interaction in solution based on enzyme-linked immunosorbent assay.

Authors:  G Zhuang; Y Katakura; T Omasa; M Kishimoto; K Suga
Journal:  J Biosci Bioeng       Date:  2001       Impact factor: 2.894

2.  Simulation model for predicting limit of detection and range of quantitation of competitive enzyme-linked immunosorbent assay.

Authors:  Dong Hwan Choi; Yoshio Katakura; Rieko Matsuda; Yuzuru Hayashi; Kazuaki Ninomiya; Suteaki Shioya
Journal:  J Biosci Bioeng       Date:  2007-05       Impact factor: 2.894

Review 3.  Biomolecular interaction analysis: affinity biosensor technologies for functional analysis of proteins.

Authors:  M Malmqvist; R Karlsson
Journal:  Curr Opin Chem Biol       Date:  1997-10       Impact factor: 8.822

Review 4.  Surface plasmon resonance for detection and measurement of antibody-antigen affinity and kinetics.

Authors:  M Malmqvist
Journal:  Curr Opin Immunol       Date:  1993-04       Impact factor: 7.486

5.  Surface plasmon resonance label-free monitoring of antibody antigen interactions in real time.

Authors:  Asta Kausaite; Martijn van Dijk; Jan Castrop; Almira Ramanaviciene; John P Baltrus; Juzefa Acaite; Arunas Ramanavicius
Journal:  Biochem Mol Biol Educ       Date:  2007-01       Impact factor: 1.160

6.  Highly specific detection of interleukin-6 (IL-6) protein using combination tapered fiber-optic biosensor dip-probe.

Authors:  Rakesh Kapoor; Chun-Wei Wang
Journal:  Biosens Bioelectron       Date:  2009-02-10       Impact factor: 10.618
  6 in total
  1 in total

Review 1.  Label-free cytokine micro- and nano-biosensing towards personalized medicine of systemic inflammatory disorders.

Authors:  Pengyu Chen; Nien-Tsu Huang; Meng-Ting Chung; Timothy T Cornell; Katsuo Kurabayashi
Journal:  Adv Drug Deliv Rev       Date:  2015-09-25       Impact factor: 15.470
  1 in total

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