Literature DB >> 21784906

D1/D2 domain of large-subunit ribosomal DNA for differentiation of Orpinomyces spp.

Sumit S Dagar1, Sanjay Kumar, Priti Mudgil, Rameshwar Singh, Anil K Puniya.   

Abstract

This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.

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Year:  2011        PMID： 21784906      PMCID： PMC3187145          DOI： 10.1128/AEM.05441-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

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  10 in total

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