| Literature DB >> 21784062 |
Guido Angelini1, Marco Chiarini, Paolo De Maria, Antonella Fontana, Carla Gasbarri, Gabriella Siani, Diana Velluto.
Abstract
The cationic large unilamellar mixed liposomes from 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) and didodecyldimethylammonium bromide (DDAB) or dioctadecyldimethylammonium bromide (DODAB) were prepared. The influence of the addition of Triton X-100 (TX-100) or octaethylene glycol mono-n-dodecylether (C(12)E(8)) on the membrane integrity was investigated turbidimetrically. The stability of the liposomal systems was estimated by monitoring fluorimetrically at 25 °C the rate of spontaneous and surfactant-induced release of entrapped 5(6)-carboxyfluorescein (CF). In order to evaluate the interaction of the cationic DODAB guest with the host POPC membrane, the main phase transition temperatures (T(m)) were determined by electron paramagnetic resonance spectroscopy (EPR). All the results obtained show that the presence of DODAB and DDAB stabilizes the POPC liposomes. The extent of stabilization depends on the concentration and nature of the cationic guest.Entities:
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Year: 2011 PMID： 21784062 DOI： 10.1016/j.chemphyslip.2011.07.002
Source DB: PubMed Journal: Chem Phys Lipids ISSN： 0009-3084 Impact factor: 3.329