Compositions and oxidative damage of condensate, particulate and semivolatile organic compounds from gasoline exhausts.

The effects of extracts of condensate, particulate and semivolatile organic compounds from absolute gasoline exhausts on DNA single strand break, levels of malondialdehyde (MDA), carbonyl protein and activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were investigated in lungs and brains of adult Sprague-Dawley rats of both sexes. In addition, the non-conventional components of the extracts and concentrations of 13 polycyclic aromatic hydrocarbon (PAHs) in gasoline exhaust were measured by GS/MS. Extract of gasoline exhaust at different doses (5.6, 16.7 and 50.0L/kg) were given to administered animals by intratracheal instillation once a week for 4 weeks, while blank control and solvent groups were given with physiological saline and dimethyl sulfoxide (DMSO). Our results showed that gasoline exhaust increased DNA single strand break, promoted lipid peroxidation and oxidative protein damage and decreased activities of SOD in lungs and brains. While, it decreased the activities of GPx in lungs but not in brains. The present data suggested that gasoline exhaust exposure could cause oxidative damage to lung and brain of rats. That was to say that gasoline is a toxin to brain of mammals, not only to lung.