Literature DB >> 2178202

Generation and application of a monoclonal antibody raised against a recombinant cytomegalovirus-specific polypeptide.

G Jahn1, H P Harthus, M Bröker, B Borisch, B Platzer, B Plachter.   

Abstract

Procedures for diagnostics of cytomegalovirus infections include histopathology, cell culture, serology, and direct detection of viral antigens or nucleic acids within infected cells or tissues. In order to develop a new diagnostic reagent for viral antigen detection, we generated a mouse monoclonal antibody. This antibody was raised against a recombinant antigen representing part of the large phosphorylated structural protein pp150 of human cytomegalovirus. The monoclonal antibody was shown to be useful for antigen detection by immunofluorescence and immunoenzymatic staining in infected cells from cell culture as well as from infected organs. The antibody proved to be reactive even in paraffin-embedded sections from tissue specimens.

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Year:  1990        PMID： 2178202     DOI： 10.1007/BF01646545

Source DB:  PubMed          Journal:  Klin Wochenschr        ISSN： 0023-2173


  19 in total

1.  Early detection of cytomegalovirus in cell culture by a new monoclonal antibody, CCH2.

Authors:  B Zweygberg Wirgart; M Landqvist; I Hökeberg; B M Eriksson; E Olding-Stenkvist; L Grillner
Journal:  J Virol Methods       Date:  1990-02       Impact factor: 2.014

2.  Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen.

Authors:  P D Swenson; M H Kaplan
Journal:  J Clin Microbiol       Date:  1985-05       Impact factor: 5.948

3.  The diagnostic, prophylactic, and therapeutic uses of monoclonal antibodies to human cytomegalovirus.

Authors:  D Emanuel; J Peppard; J Chehimi; U Hammerling; R O'Reilly
Journal:  Transplant Proc       Date:  1987-12       Impact factor: 1.066

4.  Defined monoclonal antibodies to Escherichia coli beta-galactosidase as a tool for characterisation of recombinant expression products.

Authors:  M Bröker; H P Harthus
Journal:  FEBS Lett       Date:  1989-10-23       Impact factor: 4.124

5.  The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties.

Authors:  G Jahn; B C Scholl; B Traupe; B Fleckenstein
Journal:  J Gen Virol       Date:  1987-05       Impact factor: 3.891

6.  Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus.

Authors:  G Jahn; T Kouzarides; M Mach; B C Scholl; B Plachter; B Traupe; E Preddie; S C Satchwell; B Fleckenstein; B G Barrell
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

7.  Immunohistochemical detection of an immediate early antigen of human cytomegalovirus in normal tissues.

Authors:  C B Toorkey; D R Carrigan
Journal:  J Infect Dis       Date:  1989-11       Impact factor: 5.226

8.  Prokaryotic expression of immunogenic polypeptides of the large phosphoprotein (pp150) of human cytomegalovirus.

Authors:  B C Scholl; J Von Hintzenstern; B Borisch; B Traupe; M Bröker; G Jahn
Journal:  J Gen Virol       Date:  1988-06       Impact factor: 3.891

9.  Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucocytes.

Authors:  W van der Bij; R Torensma; W J van Son; J Anema; J Schirm; A M Tegzess; T H The
Journal:  J Med Virol       Date:  1988-06       Impact factor: 2.327

10.  [Congenital cytomegalovirus infection with fatal outcome].

Authors:  G Jahn; W Pohl; B Plachter; J Hintzenstern
Journal:  Dtsch Med Wochenschr       Date:  1988-03-18       Impact factor: 0.628
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  13 in total

1.  The tegument protein UL71 of human cytomegalovirus is involved in late envelopment and affects multivesicular bodies.

Authors:  Martin Schauflinger; Daniela Fischer; Andreas Schreiber; Meike Chevillotte; Paul Walther; Thomas Mertens; Jens von Einem
Journal:  J Virol       Date:  2011-02-02       Impact factor: 5.103

2.  Nucleocytoplasmic shuttling and CRM1-dependent MHC class I peptide presentation of human cytomegalovirus pp65.

Authors:  Nadine Frankenberg; Peter Lischka; Sandra Pepperl-Klindworth; Thomas Stamminger; Bodo Plachter
Journal:  Med Microbiol Immunol       Date:  2012-09-11       Impact factor: 3.402

3.  Characterization of monoclonal antibodies specific for the Merkel cell polyomavirus capsid.

Authors:  Diana V Pastrana; Katherine A Pumphrey; Nicolas Cuburu; Rachel M Schowalter; Christopher B Buck
Journal:  Virology       Date:  2010-07-03       Impact factor: 3.616

4.  Human Cytomegalovirus pUL47 Modulates Tegumentation and Capsid Accumulation at the Viral Assembly Complex.

Authors:  Ilaria Cappadona; Clarissa Villinger; Gabi Schutzius; Thomas Mertens; Jens von Einem
Journal:  J Virol       Date:  2015-05-06       Impact factor: 5.103

5.  Human cytomegalovirus chemokine receptor gene US28 is transcribed in latently infected THP-1 monocytes.

Authors:  P S Beisser; L Laurent; J L Virelizier; S Michelson
Journal:  J Virol       Date:  2001-07       Impact factor: 5.103

6.  Dense bodies of human cytomegalovirus induce both humoral and cellular immune responses in the absence of viral gene expression.

Authors:  S Pepperl; J Münster; M Mach; J R Harris; B Plachter
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

7.  Cell types infected in human cytomegalovirus placentitis identified by immunohistochemical double staining.

Authors:  C Sinzger; H Müntefering; T Löning; H Stöss; B Plachter; G Jahn
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1993

8.  The dominant phosphoprotein pp65 (UL83) of human cytomegalovirus is dispensable for growth in cell culture.

Authors:  S Schmolke; H F Kern; P Drescher; G Jahn; B Plachter
Journal:  J Virol       Date:  1995-10       Impact factor: 5.103

9.  Major tegument protein pp65 of human cytomegalovirus is required for the incorporation of pUL69 and pUL97 into the virus particle and for viral growth in macrophages.

Authors:  Meike Chevillotte; Sandra Landwehr; Leonhard Linta; Giada Frascaroli; Anke Lüske; Christopher Buser; Thomas Mertens; Jens von Einem
Journal:  J Virol       Date:  2008-12-30       Impact factor: 5.103

10.  Detection of cytomegalovirus antibodies by an enzyme-linked immunosorbent assay using recombinant polypeptides of the large phosphorylated tegument protein pp150.

Authors:  B Plachter; L Wieczorek; B C Scholl; R Ziegelmaier; G Jahn
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948
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