| Literature DB >> 21777693 |
Si-Yang Huang1, Jian-Dong Tang, Hui-Qun Song, Bao-Quan Fu, Min-Jun Xu, Xu-Chu Hu, Han Zhang, Ya-Biao Weng, Rui-Qing Lin, Xing-Quan Zhu.
Abstract
Clonorchiasis caused by Clonorchis sinensis is a fish-borne parasitic disease which is endemic in a number of countries. Using the sequences of the internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA (rDNA) of C. sinensis as genetic markers, a pair of C. sinensis-specific primers was designed and used to establish a specific PCR assay for the diagnosis of C. sinensis infection in humans, cats and fish. This approach allowed the specific identification of C. sinensis after optimizing amplification conditions, with no amplicons being amplified from related heterogeneous DNA samples, and sequencing of amplicons confirmed the identity of the sequences amplified. The detection limit of this assay was 1.03 pg of adult C. sinensis, 1.1 metacercariae per gram of fish filet, and a single egg in human and cat feces. The PCR assay should provide a useful tool for the diagnosis and molecular epidemiological investigation of clonorchiasis in humans and animals.Entities:
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Year: 2011 PMID： 21777693 DOI： 10.1016/j.parint.2011.07.010
Source DB: PubMed Journal: Parasitol Int ISSN： 1383-5769 Impact factor: 2.230