| Literature DB >> 21777622 |
Quan Zhang1, Zhenlong Liu, Zeyun Mi, Xiaoyu Li, Pingping Jia, Jinming Zhou, Xiao Yin, Xuefu You, Liyan Yu, Fei Guo, Jing Ma, Chen Liang, Shan Cen.
Abstract
Bone marrow stromal cell antigen 2 (BST-2, also known as Tetherin) inhibits HIV-1 release and thereby severely impairs viral replication. HIV-1 accessory protein Vpu induces the down-regulation of cell surface BST-2, and counteracts the antiviral function of BST-2. Blocking Vpu-mediated down-regulation of cell surface BST-2 is viewed as a new opportunity for developing anti-HIV drugs. In this study, we have developed a high-throughput cell-based ELISA to identify small molecules that antagonize HIV-1 Vpu function and consequently inhibit HIV-1 replication through rescuing the antiviral activity of host BST-2. This cell-ELISA shows an excellent correlation with results obtained by flow cytometry (FACS). Under optimal conditions, a Z' factor of 0.605 was achieved in a 96-well format. Together, these results demonstrate that this assay can be used to quantify the cell surface level of BST-2 and be adapted to a high-throughput screening for novel anti-HIV compounds.Entities:
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Year: 2011 PMID： 21777622 DOI： 10.1016/j.antiviral.2011.07.007
Source DB: PubMed Journal: Antiviral Res ISSN： 0166-3542 Impact factor: 5.970