BACKGROUND: We evaluated whether direct tissue matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) analysis of lipids may distinguish intrahepatic cholangiocarcinomas from adjacent normal tissue and from other adenocarcinomas that frequently metastasize to liver. METHODS: Four pairs of frozen surgical specimens of cholangiocarcinomas and adjacent normal tissue were analyzed using histology-directed, MALDI MS analysis. 2,5-dihydroxybenzoic acid / α-cyano-4-hydroxycinnamic acid were manually deposited on tumor-rich areas, and mass spectra were acquired using a MALDI-time of flight instrument. RESULTS: Cholangiocarcinomas and adjacent normal tissue samples demonstrated different lipid profiles, as evidenced by permutation P value<0.05 for the cross-validated misclassification rate. Cancer-associated lipid alteration was similar between cholangiocarcinomas and pancreatic cancers, but not between cholangiocarcinomas and colorectal cancers. Baseline lipid profiles were different between cholangiocarcinoma and colorectal cancers. CONCLUSIONS: MALDI MS analysis of lipid distinguishes cancerous epithelium of cholangiocarcinoma from adjacent normal tissue, and between cholangiocarcinomas and colorectal cancers.
BACKGROUND: We evaluated whether direct tissue matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) analysis of lipids may distinguish intrahepatic cholangiocarcinomas from adjacent normal tissue and from other adenocarcinomas that frequently metastasize to liver. METHODS: Four pairs of frozen surgical specimens of cholangiocarcinomas and adjacent normal tissue were analyzed using histology-directed, MALDI MS analysis. 2,5-dihydroxybenzoic acid / α-cyano-4-hydroxycinnamic acid were manually deposited on tumor-rich areas, and mass spectra were acquired using a MALDI-time of flight instrument. RESULTS:Cholangiocarcinomas and adjacent normal tissue samples demonstrated different lipid profiles, as evidenced by permutation P value<0.05 for the cross-validated misclassification rate. Cancer-associated lipid alteration was similar between cholangiocarcinomas and pancreatic cancers, but not between cholangiocarcinomas and colorectal cancers. Baseline lipid profiles were different between cholangiocarcinoma and colorectal cancers. CONCLUSIONS: MALDI MS analysis of lipid distinguishes cancerous epithelium of cholangiocarcinoma from adjacent normal tissue, and between cholangiocarcinomas and colorectal cancers.
Authors: Pierre-Maxence Vaysse; Heike I Grabsch; Mari F C M van den Hout; Marc H A Bemelmans; Ron M A Heeren; Steven W M Olde Damink; Tiffany Porta Siegel Journal: Lab Invest Date: 2021-01-22 Impact factor: 5.662
Authors: Jae Hun Jung; Min Young Lee; Do-Young Choi; Jae Won Lee; Sungyong You; Kye Young Lee; Jayoung Kim; Kwang Pyo Kim Journal: Proteomics Date: 2015-01-16 Impact factor: 3.984
Authors: Natalia L Starodubtseva; Vitaliy V Chagovets; Maria E Nekrasova; Niso M Nazarova; Alisa O Tokareva; Olga V Bourmenskaya; Djamilja I Attoeva; Eugenii N Kukaev; Dmitriy Y Trofimov; Vladimir E Frankevich; Gennady T Sukhikh Journal: Metabolites Date: 2022-05-31
Authors: Jiayi Li; Qiao Chen; Lei Guo; Ji Li; Bao Jin; Xiangan Wu; Yue Shi; Haifeng Xu; Yongchang Zheng; Yingyi Wang; Shunda Du; Zhili Li; Xin Lu; Xinting Sang; Yilei Mao Journal: Cancer Manag Res Date: 2022-09-26 Impact factor: 3.602