Literature DB >> 21773923

Analysis of microtubule assembly kinetics using turbidimetry.

Felicia Gaskin1.   

Abstract

Turbidity measurements are rapid and reliable methods to follow the effects of drugs, proteins, nucleotides, metals, and other factors on the assembly kinetics of tubulin into microtubules in vitro and have been in use since 1974. Improvements in spectrophotometers and software have resulted in the use of less protein, and more curves can be analyzed continuously and almost simultaneously.

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Year:  2011        PMID: 21773923     DOI: 10.1007/978-1-61779-252-6_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  6 in total

1.  Factor quinolinone inhibitors disrupt spindles and multiple LSF (TFCP2)-protein interactions in mitosis, including with microtubule-associated proteins.

Authors:  Sarah A Yunes; Jennifer L S Willoughby; Julian H Kwan; Jessica M Biagi; Niranjana Pokharel; Hang Gyeong Chin; Emily A York; Kuan-Chung Su; Kelly George; Jagesh V Shah; Andrew Emili; Scott E Schaus; Ulla Hansen
Journal:  PLoS One       Date:  2022-06-15       Impact factor: 3.752

2.  Novel function of N-acetyltransferase for microtubule stability and JNK signaling in Drosophila organ development.

Authors:  Jung-Wan Mok; Kwang-Wook Choi
Journal:  Proc Natl Acad Sci U S A       Date:  2021-01-26       Impact factor: 12.779

3.  IPP51, a chalcone acting as a microtubule inhibitor with in vivo antitumor activity against bladder carcinoma.

Authors:  Véronique Martel-Frachet; Michelle Keramidas; Alessandra Nurisso; Salvatore DeBonis; Claire Rome; Jean-Luc Coll; Ahcène Boumendjel; Dimitrios A Skoufias; Xavier Ronot
Journal:  Oncotarget       Date:  2015-06-10

4.  Ensconsin/Map7 promotes microtubule growth and centrosome separation in Drosophila neural stem cells.

Authors:  Emmanuel Gallaud; Renaud Caous; Aude Pascal; Franck Bazile; Jean-Philippe Gagné; Sébastien Huet; Guy G Poirier; Denis Chrétien; Laurent Richard-Parpaillon; Régis Giet
Journal:  J Cell Biol       Date:  2014-03-31       Impact factor: 10.539

5.  Establishing a High-content Analysis Method for Tubulin Polymerization to Evaluate Both the Stabilizing and Destabilizing Activities of Compounds.

Authors:  Chi Shing Sum; Debra Nickischer; Ming Lei; Andrea Weston; Litao Zhang; Liang Schweizer
Journal:  Curr Chem Genom Transl Med       Date:  2014-02-07

6.  A novel microtubule inhibitor 4SC-207 with anti-proliferative activity in taxane-resistant cells.

Authors:  Elena Bausch; Hella Kohlhof; Svetlana Hamm; Rolf Krauss; Roland Baumgartner; Lucia Sironi
Journal:  PLoS One       Date:  2013-11-06       Impact factor: 3.240

  6 in total

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