Literature DB >> 21773918

Purification of tubulin from porcine brain.

Christopher Gell1, Claire T Friel, Barbara Borgonovo, David N Drechsel, Anthony A Hyman, Jonathon Howard.   

Abstract

Microtubules, polymers of the heterodimeric protein αβ-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.

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Year:  2011        PMID： 21773918     DOI： 10.1007/978-1-61779-252-6_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  28 in total

1.  XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules.

Authors:  Simone B Reber; Johannes Baumgart; Per O Widlund; Andrei Pozniakovsky; Jonathon Howard; Anthony A Hyman; Frank Jülicher
Journal:  Nat Cell Biol       Date:  2013-08-25       Impact factor: 28.824

Review 2.  From isolated structures to continuous networks: A categorization of cytoskeleton-based motile engineered biological microstructures.

Authors:  Rachel Andorfer; Joshua D Alper
Journal:  Wiley Interdiscip Rev Nanomed Nanobiotechnol       Date:  2019-02-11

3.  Displacement-weighted velocity analysis of gliding assays reveals that Chlamydomonas axonemal dynein preferentially moves conspecific microtubules.

Authors:  Joshua D Alper; Miguel Tovar; Jonathon Howard
Journal:  Biophys J       Date:  2013-05-07       Impact factor: 4.033

4.  Covalent immobilization of microtubules on glass surfaces for molecular motor force measurements and other single-molecule assays.

Authors:  Matthew P Nicholas; Lu Rao; Arne Gennerich
Journal:  Methods Mol Biol       Date:  2014

5.  Stu2, the budding yeast XMAP215/Dis1 homolog, promotes assembly of yeast microtubules by increasing growth rate and decreasing catastrophe frequency.

Authors:  Marija Podolski; Mohammed Mahamdeh; Jonathon Howard
Journal:  J Biol Chem       Date:  2014-08-29       Impact factor: 5.157

6.  Microtubule lattice spacing governs cohesive envelope formation of tau family proteins.

Authors:  Valerie Siahaan; Ruensern Tan; Tereza Humhalova; Lenka Libusova; Samuel E Lacey; Tracy Tan; Mariah Dacy; Kassandra M Ori-McKenney; Richard J McKenney; Marcus Braun; Zdenek Lansky
Journal:  Nat Chem Biol       Date:  2022-08-22       Impact factor: 16.174

7.  The growth speed of microtubules with XMAP215-coated beads coupled to their ends is increased by tensile force.

Authors:  Anastasiya Trushko; Erik Schäffer; Jonathon Howard
Journal:  Proc Natl Acad Sci U S A       Date:  2013-08-20       Impact factor: 11.205

8.  One-step purification of assembly-competent tubulin from diverse eukaryotic sources.

Authors:  Per O Widlund; Marija Podolski; Simone Reber; Joshua Alper; Marko Storch; Anthony A Hyman; Jonathon Howard; David N Drechsel
Journal:  Mol Biol Cell       Date:  2012-09-19       Impact factor: 4.138

9.  GTSE1 is a microtubule plus-end tracking protein that regulates EB1-dependent cell migration.

Authors:  Massimilano Scolz; Per O Widlund; Silvano Piazza; Debora Rosa Bublik; Simone Reber; Leticia Y Peche; Yari Ciani; Nina Hubner; Mayumi Isokane; Martin Monte; Jan Ellenberg; Anthony A Hyman; Claudio Schneider; Alexander W Bird
Journal:  PLoS One       Date:  2012-12-07       Impact factor: 3.240

10.  Mutations in Human Tubulin Proximal to the Kinesin-Binding Site Alter Dynamic Instability at Microtubule Plus- and Minus-Ends.

Authors:  Shih-Chieh Ti; Melissa C Pamula; Stuart C Howes; Christian Duellberg; Nicholas I Cade; Ralph E Kleiner; Scott Forth; Thomas Surrey; Eva Nogales; Tarun M Kapoor
Journal:  Dev Cell       Date:  2016-04-04       Impact factor: 12.270
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