Literature DB >> 21773772

Molecular identification and genotyping of Microsporidia in selected hosts.

Alexandra Valencakova1, Pavol Balent, Petra Ravaszova, Ales Horak, Miroslav Obornik, Monika Halanova, Beata Malcekova, Frantisek Novotny, Maria Goldova.   

Abstract

The work is described by microscopic analysis, the serological analysis (IFAT) and the molecular analysis of isolates from clinical samples (blood, faeces and urine) from ten domestic rabbits (Oryctolagus cuniculus), breed Maličký, four New Zealand domestic rabbits, 11 sows of breed Slo0076akian Improved White and 15 clinically healthy laboratory BALB/c mice. The aim of the study was to validate the suitability of species-unspecific primer pairs 530F and 580R for genotype determination of the Microsporidia strain and species-specific primer pairs ECUNF and ECUNR, SINTF and SINTR and EBIER1 and EBIEF1 for the determination of E ncephalitozoon cuniculi, Encephalitozoon intestinalis and Enterocytozoon bieneusi species for diagnostic purposes. Sequences of animals were compared with those from the GenBank database. In rabbits, two murine genotypes II and four canine genotypes III were identified. Genotype II was identified in mice. The Encephalitozoon intestinalis identified in the sample from swine showed no genetic heterogeneity.

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Year:  2011        PMID： 21773772     DOI： 10.1007/s00436-011-2543-9

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  24 in total

1.  Encephalitozoon cuniculi in pet rabbits.

Authors:  F M Harcourt-Brown; H K R Holloway
Journal:  Vet Rec       Date:  2003-04-05       Impact factor: 2.695

2.  First detection and genotyping of Encephalitozoon cuniculi in a new host species, gyrfalcon (Falco rusticolus).

Authors:  Beata Malcekova; Alexandra Valencakova; Lenka Luptakova; Ladislav Molnar; Petra Ravaszova; Frantisek Novotny
Journal:  Parasitol Res       Date:  2010-12-22       Impact factor: 2.289

3.  Extraction of microsporidial DNA from modified trichrome-stained clinical slides and subsequent species identification using PCR sequencing.

Authors:  K S Chan; T H Koh
Journal:  Parasitology       Date:  2008-04-28       Impact factor: 3.234

4.  Staining of microsporidian spores by optical brighteners with remarks on the use of brighteners for the diagnosis of AIDS associated human microsporidioses.

Authors:  J Vávra; R Dahbiová; W S Hollister; E U Canning
Journal:  Folia Parasitol (Praha)       Date:  1993       Impact factor: 2.122

5.  Microsporidia in exotic birds: intermittent spore excretion of Encephalitozoon spp. in naturally infected budgerigars (Melopsittacus undulatus).

Authors:  Bohumil Sak; Denisa Kasicková; Martin Kvác; Dana Kvetonová; Oleg Ditrich
Journal:  Vet Parasitol       Date:  2009-11-24       Impact factor: 2.738

6.  Encephalitozoonosis in pet rabbits (Oryctolagus cuniculus): pathohistological findings in animals with latent infection versus clinical manifestation.

Authors:  Jacqueline Csokai; Andrea Gruber; Frank Künzel; Alexander Tichy; Anja Joachim
Journal:  Parasitol Res       Date:  2008-11-08       Impact factor: 2.289

7.  Septata intestinalis frequently isolated from stool of AIDS patients with a new cultivation method.

Authors:  T van Gool; E U Canning; H Gilis; M A van den Bergh Weerman; J K Eeftinck Schattenkerk; J Dankert
Journal:  Parasitology       Date:  1994-09       Impact factor: 3.234

8.  Comparison of three staining methods for detecting microsporidia in fluids.

Authors:  E S Didier; J M Orenstein; A Aldras; D Bertucci; L B Rogers; F A Janney
Journal:  J Clin Microbiol       Date:  1995-12       Impact factor: 5.948

Review 9.  Utility of microsporidian rRNA in diagnosis and phylogeny: a review.

Authors:  L M Weiss; X Zhu; A Cali; H B Tanowitz; M Wittner
Journal:  Folia Parasitol (Praha)       Date:  1994       Impact factor: 2.122

10.  PCR detection of Neospora caninum, Toxoplasma gondii and Encephalitozoon cuniculi in brains of wild carnivores.

Authors:  L Hůrková; D Modrý
Journal:  Vet Parasitol       Date:  2006-02-10       Impact factor: 2.738
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  6 in total

1.  Comparative analysis of detection limits and specificity of molecular diagnostic markers for three pathogens (Microsporidia, Nosema spp.) in the key pollinators Apis mellifera and Bombus terrestris.

Authors:  Silvio Erler; Stefanie Lommatzsch; H Michael G Lattorff
Journal:  Parasitol Res       Date:  2011-09-17       Impact factor: 2.289

2.  High diversity of human-pathogenic Enterocytozoon bieneusi genotypes in swine in northeast China.

Authors:  Wei Li; Ruinan Diao; Jinping Yang; Lihua Xiao; Yixin Lu; Yijing Li; Mingxin Song
Journal:  Parasitol Res       Date:  2014-01-18       Impact factor: 2.289

3.  Chronic Infections in Mammals Due to Microsporidia.

Authors:  Bohumil Sak; Martin Kváč
Journal:  Exp Suppl       Date:  2022

4.  Genetic variation of mini- and microsatellites and a clonal structure in Enterocytozoon bieneusi population in foxes and raccoon dogs and population differentiation of the parasite between fur animals and humans.

Authors:  Wei Li; Qiang Wan; Qinlei Yu; Yuqi Yang; Wei Tao; Yanxue Jiang; Lihua Xiao
Journal:  Parasitol Res       Date:  2016-04-20       Impact factor: 2.289

5.  Identification of the microsporidian Encephalitozoon cuniculi as a new target of the IFNγ-inducible IRG resistance system.

Authors:  Marialice da Fonseca Ferreira-da-Silva; Marialice da Fonseca Ferreira-da-Silva; Helen Maria Springer-Frauenhoff; Wolfgang Bohne; Jonathan C Howard
Journal:  PLoS Pathog       Date:  2014-10-30       Impact factor: 6.823

6.  Comparison of the Concentration of Encephalitozoon cuniculi Genotypes I and III in Inflammatory Foci Under Experimental Conditions.

Authors:  Bohumil Sak; Nikola Holubová; Dana Květoňová; Lenka Hlásková; Jana Tinavská; Marta Kicia; Żaneta Zajączkowska; Martin Kváč
Journal:  J Inflamm Res       Date:  2022-04-26
  6 in total

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