Literature DB >> 2177079

An in vitro system for testing Bacillus thuringiensis toxins: the lawn assay.

J L Gringorten1, D P Witt, R E Milne, P G Fast, S S Sohi, K van Frankenhuyzen.   

Abstract

A cell assay system was developed that allows Bacillus thuringiensis delta-endotoxins activated at high pH (10.5) to be tested in vitro without causing alkaline injury to target cells. The assay is carried out on a lawn of gel-suspended cells, requires only 1 microliter of sample per dose, and is quantitative, rapid, and sensitive. The threshold dose for toxicity of B. thuringiensis subsp. kurstaki HD-73 with IPRI-CF-1 cells was 24 pg protein. The assay is also very useful for identifying antibodies which inhibit toxicity and for detecting beta-exotoxin.

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Year:  1990        PMID： 2177079     DOI： 10.1016/0022-2011(90)90106-g

Source DB:  PubMed          Journal:  J Invertebr Pathol        ISSN： 0022-2011            Impact factor:   2.841


  9 in total

1.  Specificity of Activated CryIA Proteins from Bacillus thuringiensis subsp. kurstaki HD-1 for Defoliating Forest Lepidoptera.

Authors:  K van Frankenhuyzen; J L Gringorten; R E Milne; D Gauthier; M Pusztai; R Brousseau; L Masson
Journal:  Appl Environ Microbiol       Date:  1991-06       Impact factor: 4.792

2.  Cytotoxic activity of Bacillus thuringiensis Cry proteins on mammalian cells transfected with cadherin-like Cry receptor gene of Bombyx mori (silkworm).

Authors:  Yoko Tsuda; Fumiki Nakatani; Keiko Hashimoto; Satoshi Ikawa; Chikako Matsuura; Takashi Fukada; Kenji Sugimoto; Michio Himeno
Journal:  Biochem J       Date:  2003-02-01       Impact factor: 3.857

3.  Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC delta-endotoxin affects insecticidal specificity.

Authors:  G P Smith; D J Ellar
Journal:  Biochem J       Date:  1994-09-01       Impact factor: 3.857

4.  Insecticidal properties of a crystal protein gene product isolated from Bacillus thuringiensis subsp. kenyae.

Authors:  L Masson; W J Moar; K van Frankenhuyzen; M Bossé; R Brousseau
Journal:  Appl Environ Microbiol       Date:  1992-02       Impact factor: 4.792

5.  Activity spectra of Bacillus thuringiensis delta-endotoxins against eight insect cell lines.

Authors:  J L Gringorten; S S Sohi; L Masson
Journal:  In Vitro Cell Dev Biol Anim       Date:  1999-05       Impact factor: 2.416

6.  Mutagenic analysis of a conserved region of domain III in the Cry1Ac toxin of Bacillus thuringiensis.

Authors:  Luke Masson; Bruce E Tabashnik; Alberto Mazza; Gabrielle Préfontaine; Léna Potvin; Roland Brousseau; Jean-Louis Schwartz
Journal:  Appl Environ Microbiol       Date:  2002-01       Impact factor: 4.792

7.  Lepidopteran-specific crystal toxins from Bacillus thuringiensis form cation- and anion-selective channels in planar lipid bilayers.

Authors:  J L Schwartz; L Garneau; D Savaria; L Masson; R Brousseau; E Rousseau
Journal:  J Membr Biol       Date:  1993-02       Impact factor: 1.843

8.  Cry9Ca1 Toxin, a Bacillus thuringiensis Insecticidal Crystal Protein with High Activity against the Spruce Budworm (Choristoneura fumiferana).

Authors:  K Van Frankenhuyzen; L Gringorten; D Gauthier
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

9.  Ionic permeabilities induced by Bacillus thuringiensis in Sf9 cells.

Authors:  V Vachon; M J Paradis; M Marsolais; J L Schwartz; R Laprade
Journal:  J Membr Biol       Date:  1995-11       Impact factor: 1.843
  9 in total

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