PURPOSE: The effects of radiation +/- hypogravity on immunologic function were investigated using the Partial Weight Suspension (PWS) model ( Wagner et al. 2010 ). MATERIALS AND METHODS: Mice were exposed to 0.5, 1, or 2 Gray (Gy) dose of gamma radiation and then placed in the PWS system for 4, 24, 48 hours, or 4 days. Spleens were excised and white blood cells were prepared for flow cytometry analyses. RESULTS: The combination of PWS + radiation (1 and 2 Gy doses only) resulted in decreased cell viability at the 24 h (∼16% decrease), 48 h (∼20% decrease), and 4 day (∼20% decrease) time points, compared to the PWS (no radiation) and no treatment (non-suspended, non-irradiated) groups. The T lymphocyte (thymus-derived) population increased by ∼10% (24 h, 48 h, and 4 day time points), while the B lymphocyte (bursal or bone marrow-derived) population decreased by ∼10% (at all time points examined), when mice were exposed to PWS + radiation (2 Gy dose only), compared to the PWS or no treatment groups. T cell activation was observed in the PWS group and the 0.5 Gy +/- PWS groups at the 4 and 24 h time points, compared to the no treatment group. However, T cell activation was significantly suppressed (∼85%) at the acute time points in the 2 Gy +/- PWS groups, comparable to the no treatment group. CONCLUSIONS: Ionizing radiation in the absence and presence of simulated hypogravity results in acute lymphocyte dysfunction and compromised immune response.
PURPOSE: The effects of radiation +/- hypogravity on immunologic function were investigated using the Partial Weight Suspension (PWS) model ( Wagner et al. 2010 ). MATERIALS AND METHODS:Mice were exposed to 0.5, 1, or 2 Gray (Gy) dose of gamma radiation and then placed in the PWS system for 4, 24, 48 hours, or 4 days. Spleens were excised and white blood cells were prepared for flow cytometry analyses. RESULTS: The combination of PWS + radiation (1 and 2 Gy doses only) resulted in decreased cell viability at the 24 h (∼16% decrease), 48 h (∼20% decrease), and 4 day (∼20% decrease) time points, compared to the PWS (no radiation) and no treatment (non-suspended, non-irradiated) groups. The T lymphocyte (thymus-derived) population increased by ∼10% (24 h, 48 h, and 4 day time points), while the B lymphocyte (bursal or bone marrow-derived) population decreased by ∼10% (at all time points examined), when mice were exposed to PWS + radiation (2 Gy dose only), compared to the PWS or no treatment groups. T cell activation was observed in the PWS group and the 0.5 Gy +/- PWS groups at the 4 and 24 h time points, compared to the no treatment group. However, T cell activation was significantly suppressed (∼85%) at the acute time points in the 2 Gy +/- PWS groups, comparable to the no treatment group. CONCLUSIONS:Ionizing radiation in the absence and presence of simulated hypogravity results in acute lymphocyte dysfunction and compromised immune response.
Authors: Erika B Wagner; Nicholas P Granzella; Hiroaki Saito; Dava J Newman; Laurence R Young; Mary L Bouxsein Journal: J Appl Physiol (1985) Date: 2010-06-03
Authors: E H Kajioka; C Gheorghe; M L Andres; G A Abell; J Folz-Holbeck; J M Slater; G A Nelson; D S Gridley Journal: In Vivo Date: 1999 Nov-Dec Impact factor: 2.155
Authors: Michael J Pecaut; Gregory A Nelson; Luanne L Peters; Paul J Kostenuik; Ted A Bateman; Sean Morony; Louis S Stodieck; David L Lacey; Steven J Simske; Daila S Gridley Journal: J Appl Physiol (1985) Date: 2003-01-03
Authors: A-S Schreurs; Y Shirazi-Fard; M Shahnazari; J S Alwood; T A Truong; C G T Tahimic; C L Limoli; N D Turner; B Halloran; R K Globus Journal: Sci Rep Date: 2016-02-11 Impact factor: 4.379
Authors: Jenine K Sanzari; Ana L Romero-Weaver; Gabrielle James; Gabriel Krigsfeld; Liyong Lin; Eric S Diffenderfer; Ann R Kennedy Journal: PLoS One Date: 2013-08-14 Impact factor: 3.240
Authors: Jeffery C Chancellor; Rebecca S Blue; Keith A Cengel; Serena M Auñón-Chancellor; Kathleen H Rubins; Helmut G Katzgraber; Ann R Kennedy Journal: NPJ Microgravity Date: 2018-04-03 Impact factor: 4.415