| Literature DB >> 21769623 |
Gen-Ichiro Uechi1, Hiromu Toma, Takeshi Arakawa, Yoshiya Sato.
Abstract
The sea anemone Actineria villosa expresses a lethal protein toxin. We isolated a novel 120-kDa protein, Avt120, from partially purified toxin and found it to possess extremely strong lethal activity. The 3,453-bp Avt120 gene translates to a 995-amino acid protein. The 50% lethal dose (LD(50)) of purified Avt120 in mice was 85.17 ng. Among several tested cell lines, Colo205 cells were most sensitive to Avt120: 50% of them were damaged by 38.4 ng/mL Avt120. Avt120 exerted ATP degradation activity (10 μmol ATP h(-1) mg(-1)), which was strongly inhibited by ganglioside GM1 to decrease the cytotoxicity of Avt120.Entities:
Mesh：
Substances：
Year: 2011 PMID： 21769623 DOI： 10.1007/s10930-011-9347-8
Source DB: PubMed Journal: Protein J ISSN： 1572-3887 Impact factor: 2.371