| Literature DB >> 21769605 |
Sathiyamoorthy Subramaniyam1, Ramya Mathiyalagan, In Jun Gyo, Lee Bum-Soo, Lee Sungyoung, Yang Deok Chun.
Abstract
Gynosaponins (Gypenosides) are major phyto-chemicals in Gynostemma pentaphyllum (Thunb.), with similarities to the ginsenosides present in Panax ginseng. Gynosaponins are classified as terpenoid compounds. In G. pentaphyllum, 25% of the total gynosaponins are similar to ginsenosides. In this study, we analyzed the transcriptional levels of the G. pentaphyllum genome to identify secondary metabolite genes. The complete transcriptomes for the roots and leaves were obtained using a GS-FLX pyro-sequencer. In total, we obtained 265,340 and all reads were well annotated according to biological databases. Using insilico analysis, 84% of sequence were well annotated and we obtained most of the secondary metabolite genes that represent mono-, di-, tri- and sesquiterpenoids. From our EST, most of the terpenoid genes were noted, among those few similar genes were studied in P. ginseng and these transcripts will help to characterize more triterpenoid genes in G. pentaphyllum. Also help to compare P. ginseng and G. pentaphyllum at transcriptome level.Entities:
Mesh：
Substances：
Year: 2011 PMID： 21769605 DOI： 10.1007/s00299-011-1114-y
Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570