A new analysis of testicular proteins through integrative bioinformatics.

The testis is the male gonad responsible for spermatogenesis and steroidogenesis. Much remains to be known about the control of these events. In this study, we performed a new bioinformatic enrichment analysis of human testicular proteins selected from a protein database. Integrated function and pathway analyses were performed by Database for Annotation, Visualization and Integrated Discovery and Ingenuity Pathway Analysis programmes, and significant features were found to be clustered. Protein membrane organization and gene density on chromosomes were analyzed and discussed. The analysis could provide a basis for the understanding of testicular physiology and function, and facilitating biological interpretation of testicular functions in a network context.