| Literature DB >> 21763623 |
Tristan R McKay1, Maria V Camarasa, Banu Iskender, Jinpei Ye, Nicola Bates, Duncan Miller, Jayne C Fitzsimmons, Daniel Foxler, Maureen Mee, Tyson V Sharp, John Aplin, Daniel R Brison, Susan J Kimber.
Abstract
We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.Entities:
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Year: 2011 PMID： 21763623 DOI： 10.1016/j.scr.2011.04.005
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020