BACKGROUND: Helicobacter pylori ClariRes assay is a novel commercially available real-time PCR assay allowing H. pylori detection and clarithromycin susceptibility testing in either gastric biopsy or stool specimens. OBJECTIVE: The aim of this study was to validate the novel biprobe real-time assay in stool specimens from 217 dyspeptic children. METHODS: DNA from gastric biopsies and stool specimens were obtained and submitted to the biprobe real time assay for H. pylori detection and clarithromycin susceptibility testing. RESULTS: The sensitivity, specificity, and test accuracy were 69, 100 and 93.9% for the detection of H. pylori infection and 83.3, 100 and 95.6%, for detection of clarithromycin resistance. CONCLUSION: This assay proved to be appropriate for H. pylori clarithromycin susceptibility testing, particularly in children populations where a high prevalence of clarithromycin-resistant strains is suspected.
BACKGROUND:Helicobacter pylori ClariRes assay is a novel commercially available real-time PCR assay allowing H. pylori detection and clarithromycin susceptibility testing in either gastric biopsy or stool specimens. OBJECTIVE: The aim of this study was to validate the novel biprobe real-time assay in stool specimens from 217 dyspeptic children. METHODS: DNA from gastric biopsies and stool specimens were obtained and submitted to the biprobe real time assay for H. pylori detection and clarithromycin susceptibility testing. RESULTS: The sensitivity, specificity, and test accuracy were 69, 100 and 93.9% for the detection of H. pyloriinfection and 83.3, 100 and 95.6%, for detection of clarithromycin resistance. CONCLUSION: This assay proved to be appropriate for H. pyloriclarithromycin susceptibility testing, particularly in children populations where a high prevalence of clarithromycin-resistant strains is suspected.
Authors: Tiago Gomes da Silva Benigno; Howard Lopes Ribeiro Junior; Orleâncio Gomes Ripardo de Azevedo; Ronald Feitosa Pinheiro; Roberta Taiane Germano de Oliveira; Felipe Silva Maciel; Edson Luiz de Oliveira; Dulciene Maria Magalhães Queiroz; Lucia Libanez Bessa Campelo Braga Journal: Rev Inst Med Trop Sao Paulo Date: 2022-07-13 Impact factor: 2.169
Authors: Rebecca Marrero Rolon; Scott A Cunningham; Jayawant N Mandrekar; Erin T Polo; Robin Patel Journal: J Clin Microbiol Date: 2021-04-20 Impact factor: 5.948