| Literature DB >> 21761301 |
Isabelle Cadron1, Toon Van Gorp, Philippe Moerman, Etienne Waelkens, Ignace Vergote.
Abstract
The evolution of ovarian cancer will highly depend on platinum sensitivity or resistance. In an attempt to better understand this mechanism of resistance, we combined laser microdissection (LMD) of ovarian tumor cells with mass spectrometric techniques. To obtain disease-specific markers we isolated ovarian tumor cells with LMD without contamination of surrounding cells. Proteins were extracted by chemical lysis and subsequent peptide and protein information was gathered using surface-enhanced laser desorption/ionization-time of flight mass spectrometry.Entities:
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Year: 2011 PMID： 21761301 DOI： 10.1007/978-1-61779-163-5_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745