AIM: Porphyromonas gingivalis lipopolysaccharide (LPS) displays a significant amount of structural heterogeneity, containing both tetra- (LPS(1435/1449) ) and penta-acylated (LPS(1690) ) lipid A structures. This study investigated the effects of the two isoforms of P. gingivalis LPS on the expression of IL-6, IL-8 and TNF-α in human gingival fibroblasts (HGFs). MATERIALS AND METHODS: HGFs were stimulated with P. gingivalis LPS(1435/1449) and LPS(1690) in both dose- (1 ng-10 μg/ml) and time-dependent (2-48 h) experiments. Total RNA and protein were extracted and used for analysis of the IL-6, IL-8 and TNF-α transcripts as well as IL-6 and IL-8 proteins, by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. RESULTS: P. gingivalis LPS(1690) significantly up-regulated the mRNA and protein expression of IL-6 and IL-8, whereas P. gingivalis LPS(1435/1449) did not induce significant host response. The expression levels of IL-6 and IL-8 up-regulated by P. gingivalis LPS(1690) continuously increased with time course. In contrast, TNF-α transcript expression was up-regulated promptly by P. gingivalis LPS(1690) after 2 h of stimulation and gradually declined afterwards. CONCLUSIONS: This study suggests that P. gingivalis LPS heterogeneity may differentially modulate the pro-inflammatory cytokine expression in HGFs, which may contribute to periodontal pathogenesis.
AIM: Porphyromonas gingivalislipopolysaccharide (LPS) displays a significant amount of structural heterogeneity, containing both tetra- (LPS(1435/1449) ) and penta-acylated (LPS(1690) ) lipid A structures. This study investigated the effects of the two isoforms of P. gingivalis LPS on the expression of IL-6, IL-8 and TNF-α in human gingival fibroblasts (HGFs). MATERIALS AND METHODS: HGFs were stimulated with P. gingivalis LPS(1435/1449) and LPS(1690) in both dose- (1 ng-10 μg/ml) and time-dependent (2-48 h) experiments. Total RNA and protein were extracted and used for analysis of the IL-6, IL-8 and TNF-α transcripts as well as IL-6 and IL-8 proteins, by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. RESULTS: P. gingivalis LPS(1690) significantly up-regulated the mRNA and protein expression of IL-6 and IL-8, whereas P. gingivalis LPS(1435/1449) did not induce significant host response. The expression levels of IL-6 and IL-8 up-regulated by P. gingivalis LPS(1690) continuously increased with time course. In contrast, TNF-α transcript expression was up-regulated promptly by P. gingivalis LPS(1690) after 2 h of stimulation and gradually declined afterwards. CONCLUSIONS: This study suggests that P. gingivalis LPS heterogeneity may differentially modulate the pro-inflammatory cytokine expression in HGFs, which may contribute to periodontal pathogenesis.
Authors: Tommi Vatanen; Aleksandar D Kostic; Eva d'Hennezel; Heli Siljander; Eric A Franzosa; Moran Yassour; Raivo Kolde; Hera Vlamakis; Timothy D Arthur; Anu-Maaria Hämäläinen; Aleksandr Peet; Vallo Tillmann; Raivo Uibo; Sergei Mokurov; Natalya Dorshakova; Jorma Ilonen; Suvi M Virtanen; Susanne J Szabo; Jeffrey A Porter; Harri Lähdesmäki; Curtis Huttenhower; Dirk Gevers; Thomas W Cullen; Mikael Knip; Ramnik J Xavier Journal: Cell Date: 2016-04-28 Impact factor: 41.582
Authors: Thanuja D K Herath; Yu Wang; Chaminda J Seneviratne; Richard P Darveau; Cun-Yu Wang; Lijian Jin Journal: BMC Microbiol Date: 2013-03-30 Impact factor: 3.605
Authors: Thanuja D K Herath; Richard P Darveau; Chaminda J Seneviratne; Cun-Yu Wang; Yu Wang; Lijian Jin Journal: PLoS One Date: 2013-03-12 Impact factor: 3.240