Literature DB >> 2175179

Effects of herbimycin A and various SH-reagents on p60v-src kinase activity in vitro.

H Fukazawa1, S Mizuno, Y Uehara.   

Abstract

Herbimycin A is an antiobiotic which reverses transformation caused by src family oncogenes. It inactivates p60v-src in vitro, possibly by binding to reactive SH-group(s) of the kinase. We examined effects of various SH-reagents on p60v-src and observed that N-[p-(2-benzimidazolyl)phenyl]maleimide (BIPM) or N-(9-acridinyl)maleimide (NAM) were potent inactivators of the kinase, whereas N-ethylmaleimide (NEM) required high concentrations, and iodoacetamide was totally ineffective in reducing the kinase activity. Pretreatment of p60v-src immune-complex with NEM and iodoacetamide, however, protected the kinase from inactivation by herbimycin A, BIPM, and NAM. The results suggest that SH-group(s) to which herbimycin A binds is not essential for the kinase activity, but is positioned in the vicinity of the active center.

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Year:  1990        PMID： 2175179     DOI： 10.1016/s0006-291x(05)81053-8

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  12 in total

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7.  Conversion of epidermal growth factor (EGF) into a stimulatory ligand for A431-cell growth by herbimycin A by decreasing the level of expression of EGF receptor.

Authors:  Y Murakami; H Fukazawa; S Mizuno; Y Uehara
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8.  Accelerated degradation of 160 kDa epidermal growth factor (EGF) receptor precursor by the tyrosine kinase inhibitor herbimycin A in the endoplasmic reticulum of A431 human epidermoid carcinoma cells.

Authors:  Y Murakami; S Mizuno; Y Uehara
Journal:  Biochem J       Date:  1994-07-01       Impact factor: 3.857

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Journal:  J Biochem       Date:  2020-02-01       Impact factor: 3.387
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