BACKGROUND: Despite numerous treatments available for deteriorated cutaneous wound healing such as a diabetic foot, there is still the need for more effective therapy. Adipose-derived stem cells (ASCs) are mesenchymal stem cells, which are self-renewing and multipotent. Mesenchymal stem cells have the potential for tissue repair and regeneration. OBJECTIVE: To investigate the effects of human ASCs on the healing of cutaneous wounds in nude mice. METHODS: 15-mm round full-thickness skin defects were generated on the back of BALB/c nude mice. The mice were divided into three groups for wound coverage: (i) human ASCs-populated collagen gel, (ii) human dermal fibroblasts-populated collagen gel, and (iii) collagen gel alone. Wound contraction was prevented with a splint method. Wound size was measured 10 days after injury. At 28 days histological analysis was performed. RESULTS: Both ASCs and dermal fibroblasts accelerated wound closure, but dermal fibroblasts were more effective than ASCs. At 28 days, the dermal portion of ASCs or dermal fibroblasts wound scars were thicker than collagen gel wound scars. CONCLUSION: ASCs and dermal fibroblasts stimulate cutaneous wound healing and improve scar thickness.
BACKGROUND: Despite numerous treatments available for deteriorated cutaneous wound healing such as a diabetic foot, there is still the need for more effective therapy. Adipose-derived stem cells (ASCs) are mesenchymal stem cells, which are self-renewing and multipotent. Mesenchymal stem cells have the potential for tissue repair and regeneration. OBJECTIVE: To investigate the effects of human ASCs on the healing of cutaneous wounds in nude mice. METHODS: 15-mm round full-thickness skin defects were generated on the back of BALB/c nude mice. The mice were divided into three groups for wound coverage: (i) human ASCs-populated collagen gel, (ii) human dermal fibroblasts-populated collagen gel, and (iii) collagen gel alone. Wound contraction was prevented with a splint method. Wound size was measured 10 days after injury. At 28 days histological analysis was performed. RESULTS: Both ASCs and dermal fibroblasts accelerated wound closure, but dermal fibroblasts were more effective than ASCs. At 28 days, the dermal portion of ASCs or dermal fibroblasts wound scars were thicker than collagen gel wound scars. CONCLUSION: ASCs and dermal fibroblasts stimulate cutaneous wound healing and improve scar thickness.
Authors: Patricia A Zuk; Min Zhu; Peter Ashjian; Daniel A De Ugarte; Jerry I Huang; Hiroshi Mizuno; Zeni C Alfonso; John K Fraser; Prosper Benhaim; Marc H Hedrick Journal: Mol Biol Cell Date: 2002-12 Impact factor: 4.138
Authors: M F Pittenger; A M Mackay; S C Beck; R K Jaiswal; R Douglas; J D Mosca; M A Moorman; D W Simonetti; S Craig; D R Marshak Journal: Science Date: 1999-04-02 Impact factor: 47.728
Authors: S Fuchs; R Baffour; Y F Zhou; M Shou; A Pierre; F O Tio; N J Weissman; M B Leon; S E Epstein; R Kornowski Journal: J Am Coll Cardiol Date: 2001-05 Impact factor: 24.094
Authors: Oliver C Thamm; Panagiotis Theodorou; Ewa Stuermer; Max J Zinser; Edmund A Neugebauer; Paul C Fuchs; Paola Koenen Journal: Int Wound J Date: 2013-07-11 Impact factor: 3.315
Authors: Maroesjka Spiekman; Joris A van Dongen; Joep C Willemsen; Delia L Hoppe; Berend van der Lei; Martin C Harmsen Journal: J Tissue Eng Regen Med Date: 2017-02-03 Impact factor: 3.963
Authors: Seok Jong Hong; Sheng-Xian Jia; Ping Xie; Wei Xu; Kai P Leung; Thomas A Mustoe; Robert D Galiano Journal: PLoS One Date: 2013-01-31 Impact factor: 3.240
Authors: Scott J Rapp; Aaron Rumberg; Marty Visscher; David A Billmire; Ann S Schwentker; Brian S Pan Journal: Plast Reconstr Surg Glob Open Date: 2015-03-06