BACKGROUND: Due to their homology, close proximity, and opposite orientation, RHD and RHCE can exchange nucleotides giving rise to variant alleles. Some of these variants encode the so-called partial phenotypes. The DIII partial D category has been subdivided into DIIIa, DIIIb, DIIIc, DIII Type 4, DIII Type 6, and DIII Type 7. During DNA-based screening tests, we identified a second example of DIII Type 7 in a Dce donor from South Africa. Our study describes hemagglutination tests on this sample and raises a question regarding the molecular basis of the originally defined DIIIb category. STUDY DESIGN AND METHODS: Hemagglutination and DNA testing were performed by standard techniques. RESULTS: Red blood cells from this DIII Type 7 donor typed D+C-E-c+e+G-, DAK+ and did not react with anti-D made by people with the DIII phenotype. The allele is RHD*DIII 150C, 178C, 201A, 203A, 307C, 410T, 455C, 602G, 667G. CONCLUSIONS: Based on the serotype and ethnicity (black African), it is likely that DIII Type 7 is the originally defined DIIIb category.
BACKGROUND: Due to their homology, close proximity, and opposite orientation, RHD and RHCE can exchange nucleotides giving rise to variant alleles. Some of these variants encode the so-called partial phenotypes. The DIII partial D category has been subdivided into DIIIa, DIIIb, DIIIc, DIII Type 4, DIII Type 6, and DIII Type 7. During DNA-based screening tests, we identified a second example of DIII Type 7 in a Dcedonor from South Africa. Our study describes hemagglutination tests on this sample and raises a question regarding the molecular basis of the originally defined DIIIb category. STUDY DESIGN AND METHODS: Hemagglutination and DNA testing were performed by standard techniques. RESULTS: Red blood cells from this DIII Type 7 donor typed D+C-E-c+e+G-, DAK+ and did not react with anti-D made by people with the DIII phenotype. The allele is RHD*DIII 150C, 178C, 201A, 203A, 307C, 410T, 455C, 602G, 667G. CONCLUSIONS: Based on the serotype and ethnicity (black African), it is likely that DIII Type 7 is the originally defined DIIIb category.
Authors: F F Wagner; A Frohmajer; B Ladewig; N I Eicher; C B Lonicer; T H Müller; M H Siegel; W A Flegel Journal: Blood Date: 2000-04-15 Impact factor: 22.113
Authors: P A Apoil; M E Reid; G Halverson; I Mouro; Y Colin; F Roubinet; J P Cartron; A Blancher Journal: Br J Haematol Date: 1997-08 Impact factor: 6.998
Authors: Martine G H M Grootkerk-Tax; Joyce D van Wintershoven; Peter C Ligthart; Dick J van Rhenen; C Ellen van der Schoot; Petra A Maaskant-van Wijk Journal: Transfusion Date: 2006-04 Impact factor: 3.157
Authors: Franz F Wagner; Birgit Ladewig; Katharina S Angert; Guido A Heymann; Nicole I Eicher; Willy A Flegel Journal: Blood Date: 2002-07-01 Impact factor: 22.113