Literature DB >> 2174298

Flow cytometric detection of bovine viral diarrhea virus in peripheral blood leukocytes of persistently infected cattle.

P Qvist1, B Aasted, B Bloch, A Meyling, L Rønsholt, H Houe.   

Abstract

Flow cytometry was investigated for detection of bovine viral diarrhea virus (BVDV) in peripheral blood mononuclear leukocytes of persistently infected cattle. The mononuclear leukocytes were purified by sedimentation in a gradient of Ficoll-Paque, fixed, permeabilized, and then labelled by indirect immunofluorescence using biotinylated immunoglobulins from a porcine antiserum to BVDV. Flow cytometric analysis of blood samples obtained from persistently infected cattle revealed virus in 3.0-21.0% (mean +/- SD, 11.2% +/- 6.4%) of the mononuclear leukocytes. Fluorescent cells were not observed in controls. Flow cytometric detection of BVDV in blood cells of persistently infected bovines is a rapid and objective technique which does not require cell culture facilities.

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Year:  1990        PMID： 2174298      PMCID： PMC1255695     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  14 in total

1.  Quantitative measurement of SB 40 T-antigen production.

Authors:  M Horan; P K Horan; C A Williams
Journal:  Exp Cell Res       Date:  1975-03-15       Impact factor: 3.905

2.  Analysis of intracellular antigens by flow cytometry.

Authors:  J W Jacobberger; D Fogleman; J M Lehman
Journal:  Cytometry       Date:  1986-07

3.  [Production of high titrating antisera against BVD virus from swine and its use for direct immunofluorescence and immunoperoxidase technics].

Authors:  J M Hyera; J Dahle; B Liess; V Moennig; H R Frey
Journal:  Dtsch Tierarztl Wochenschr       Date:  1987 Nov-Dec

4.  Frequency of association of noncytopathic bovine viral diarrhea virus with mononuclear leukocytes from persistently infected cattle.

Authors:  S R Bolin; J M Sacks; S V Crowder
Journal:  Am J Vet Res       Date:  1987-10       Impact factor: 1.156

5.  Fluorescence-activated separation of cervical abnormal cells using herpesvirus antigenic markers.

Authors:  L Aurelian; P K Gupta; J K Frost; N B Rosenshein; C C Smith; H W Tyrer; J M Mantione; C D Albright
Journal:  Anal Quant Cytol       Date:  1979 Jul-Aug

6.  The use of avidin-biotin interaction in immunoenzymatic techniques.

Authors:  J L Guesdon; T Ternynck; S Avrameas
Journal:  J Histochem Cytochem       Date:  1979-08       Impact factor: 2.479

7.  Demonstration of bovine viral diarrhoea virus in peripheral blood mononuclear cells of persistently infected, clinically normal cattle.

Authors:  H Bielefeldt Ohmann; L Rønsholt; B Bloch
Journal:  J Gen Virol       Date:  1987-07       Impact factor: 3.891

8.  Effects of bovine viral diarrhea virus on the percentages and absolute numbers of circulating B and T lymphocytes in cattle.

Authors:  S R Bolin; A W McClurkin; M F Coria
Journal:  Am J Vet Res       Date:  1985-04       Impact factor: 1.156

9.  Bovine virus diarrhoea-mucosal disease infection in cattle.

Authors:  S J Duffell; J W Harkness
Journal:  Vet Rec       Date:  1985-09-07       Impact factor: 2.695

10.  SV40-infected muntjac cells: cell cycle kinetics, cell ploidy and T antigen concentration.

Authors:  E L Gershey
Journal:  Cytometry       Date:  1980-07
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  3 in total

1.  Comparison of flow cytometry and virus isolation in cell culture for identification of cattle persistently infected with bovine viral diarrhea virus.

Authors:  P Qvist; H Houe; B Aasted; A Meyling
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

Review 2.  Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

Authors:  H M Davey; D B Kell
Journal:  Microbiol Rev       Date:  1996-12

3.  "Self" and "nonself" manipulation of interferon defense during persistent infection: bovine viral diarrhea virus resists alpha/beta interferon without blocking antiviral activity against unrelated viruses replicating in its host cells.

Authors:  Matthias Schweizer; Philippe Mätzener; Gabriela Pfaffen; Hanspeter Stalder; Ernst Peterhans
Journal:  J Virol       Date:  2006-07       Impact factor: 5.103
  3 in total

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