Literature DB >> 21742921

Comparison of the host specificities of two bacteroidales quantitative PCR assays used for tracking human fecal contamination.

Laurie C Van De Werfhorst1, Bram Sercu, Patricia A Holden.   

Abstract

The sewage-associated real-time quantitative PCR (qPCR) assays BacHum and HF183 SYBR were compared for specificity against local fecal sources. Both assays were equally sensitive to sewage, but BacHum showed substantially more false-positive results for cat, dog, gull, and raccoon feces.

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Mesh：

Year:  2011        PMID： 21742921      PMCID： PMC3165408          DOI： 10.1128/AEM.00239-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes.

Authors:  A E Bernhard; K G Field
Journal:  Appl Environ Microbiol       Date:  2000-04       Impact factor: 4.792

2.  A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.

Authors:  A E Bernhard; K G Field
Journal:  Appl Environ Microbiol       Date:  2000-10       Impact factor: 4.792

3.  Performance of PCR-based assays targeting Bacteroidales genetic markers of human fecal pollution in sewage and fecal samples.

Authors:  Orin C Shanks; Karen White; Catherine A Kelty; Mano Sivaganesan; Janet Blannon; Mark Meckes; Manju Varma; Richard A Haugland
Journal:  Environ Sci Technol       Date:  2010-08-15       Impact factor: 9.028

4.  Comparison of Enterococcus measurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis.

Authors:  Richard A Haugland; Shawn C Siefring; Larry J Wymer; Kristen P Brenner; Alfred P Dufour
Journal:  Water Res       Date:  2004-12-24       Impact factor: 11.236

5.  Validation of host-specific Bacteriodales 16S rRNA genes as markers to determine the origin of faecal pollution in Atlantic Rim countries of the European Union.

Authors:  Andrew H Gawler; Jean E Beecher; João Brandão; Nora M Carroll; Leonor Falcão; Michèle Gourmelon; Bartholomew Masterson; Baltazar Nunes; Jonathan Porter; Alain Rincé; Raquel Rodrigues; Martin Thorp; J Martin Walters; Wim G Meijer
Journal:  Water Res       Date:  2007-03-07       Impact factor: 11.236

6.  Storm drains are sources of human fecal pollution during dry weather in three urban southern California watersheds.

Authors:  Bram Sercu; Laurie C Van De Werfhorst; Jill Murray; Patricia A Holden
Journal:  Environ Sci Technol       Date:  2009-01-15       Impact factor: 9.028

7.  Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland.

Authors:  Siobhán Dorai-Raj; Justin O' Grady; Emer Colleran
Journal:  Water Res       Date:  2009-09-06       Impact factor: 11.236

8.  Detection and quantification of the human-specific HF183 Bacteroides 16S rRNA genetic marker with real-time PCR for assessment of human faecal pollution in freshwater.

Authors:  Sylvie Seurinck; Tom Defoirdt; Willy Verstraete; Steven D Siciliano
Journal:  Environ Microbiol       Date:  2005-02       Impact factor: 5.491

9.  Concentrations of host-specific and generic fecal markers measured by quantitative PCR in raw sewage and fresh animal feces.

Authors:  Sarah S Silkie; Kara L Nelson
Journal:  Water Res       Date:  2009-08-22       Impact factor: 11.236

10.  Identifying human and livestock sources of fecal contamination in Kenya with host-specific Bacteroidales assays.

Authors:  Marion W Jenkins; Sangam Tiwari; Mario Lorente; Charles Maina Gichaba; Stefan Wuertz
Journal:  Water Res       Date:  2009-07-26       Impact factor: 11.236
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  7 in total

1.  Level of contamination in the feces of several species at major inland pollution sources in the drainage basin of Yeoja Bay, Republic of Korea.

Authors:  Sang Hyeon Jeong; Soon Bum Shin; Ji Hee Lee; Ji Young Kwon; Hee Chung Lee; Seon-Jae Kim; Kwang Soo Ha
Journal:  Environ Monit Assess       Date:  2020-02-08       Impact factor: 2.513

2.  Distributions of Fecal Markers in Wastewater from Different Climatic Zones for Human Fecal Pollution Tracking in Australian Surface Waters.

Authors:  W Ahmed; J P S Sidhu; K Smith; D J Beale; P Gyawali; S Toze
Journal:  Appl Environ Microbiol       Date:  2015-12-18       Impact factor: 4.792

3.  Effect of Sewerage on the Contamination of Soil with Pathogenic Leptospira in Urban Slums.

Authors:  Arnau Casanovas-Massana; Fabio Neves Souza; Melanie Curry; Daiana de Oliveira; Anderson S de Oliveira; Max T Eyre; Diogo Santiago; Maísa Aguiar Santos; Rafael M R Serra; Evelyn Lopes; Barbara Ia Xavier; Peter J Diggle; Elsio A Wunder; Mitermayer G Reis; Albert I Ko; Federico Costa
Journal:  Environ Sci Technol       Date:  2021-11-12       Impact factor: 11.357

4.  Human-Associated Lachnospiraceae Genetic Markers Improve Detection of Fecal Pollution Sources in Urban Waters.

Authors:  Shuchen Feng; Melinda Bootsma; Sandra L McLellan
Journal:  Appl Environ Microbiol       Date:  2018-07-02       Impact factor: 4.792

5.  Linking fecal bacteria in rivers to landscape, geochemical, and hydrologic factors and sources at the basin scale.

Authors:  Marc P Verhougstraete; Sherry L Martin; Anthony D Kendall; David W Hyndman; Joan B Rose
Journal:  Proc Natl Acad Sci U S A       Date:  2015-08-03       Impact factor: 11.205

6.  Distribution and abundance of human-specific Bacteroides and relation to traditional indicators in an urban tropical catchment.

Authors:  J P Nshimyimana; E Ekklesia; P Shanahan; L H C Chua; J R Thompson
Journal:  J Appl Microbiol       Date:  2014-02-25       Impact factor: 3.772

7.  High levels of sewage contamination released from urban areas after storm events: A quantitative survey with sewage specific bacterial indicators.

Authors:  Hayley T Olds; Steven R Corsi; Deborah K Dila; Katherine M Halmo; Melinda J Bootsma; Sandra L McLellan
Journal:  PLoS Med       Date:  2018-07-24       Impact factor: 11.069
  7 in total

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