Simplified sequence-specific oligonucleotide-based polymerase chain reaction protocol to characterize human major histocompatibility complex A*02 and A*24 specificities.

Major histocompatibility complex (MHC) multimer technology is used in studies of high scientific and clinical interest for the identification, analysis, purification, and adoptive transfer of virus-specific T cells. MHC peptide multimers are usually specific for MHC A*02 or A*24 specificities because both specificities exhibit a high worldwide frequency. However, commercially available typing methods perform complete typing instead of browsing for these prevailing specificities. In this study we demonstrate an easy and accessible polymerase chain reaction-based method to accurately identify A*02 and A*24 samples in a cost-effective way.