| Literature DB >> 21741165 |
Jian Wang1, Jingjing Wu, Jianguo Hong, Rong Chen, Kesen Xu, Weibo Niu, Cheng Peng, Enyu Liu, Jiayong Wang, Song Liu, Michael Agrez, Jun Niu.
Abstract
Recently published studies have suggested that integrin trafficking is necessary to support cell migration, but the role of internalization and recycling of integrin αvβ6 in colon cancer cells remained unclear. In our study, we demonstrated the existence of the integrin cycle and found that inhibition of ERK2 phosphorylation by PD98059 or deletion of the ERK2 direct binding site on the β6 cytoplasmic domain could interrupt the internalization of integrin αvβ6, but had no effect on its recycling. Furthermore, integrin αvβ6 trafficking played a key role in the migration of colon cancer cells towards fibronectin. Activation of PKC significantly accelerated the internalization and recycling of integrin αvβ6, which could facilitate rapid redistribution of integrin αvβ6 and increase cell motility. When colon cancer cells became crowded, the increase in αvβ6 levels at the cell surface was not accompanied by a change in total αvβ6 expression in cell lysates. This change may be due to a redistribution of αvβ6 in cell microstructures and a rapid cellular response towards the demands of migration. CrownEntities:
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Year: 2011 PMID： 21741165 DOI： 10.1016/j.canlet.2011.06.025
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679