Liang Li1, Zhe Zhang, Bin Li, Fei Gao, Liaoqing Li, Jost B Jonas. 1. Beijing Institute of Ophthalmology, Beijing Tongren Ophthalmic Center, Capital University of Medical Science, 17 Hougou Lane, Chongnei Street, Beijing, 100005, China.
Abstract
BACKGROUND: Since the E-cadherin-catenin complex is of importance in the cancerogenesis of epithelial tumors, we examined the expression of E-cadherin and β-catenin in sebaceous adenocarcinomas of the eyelid and correlated the results with clinicopathological parameters. METHODS: The study included histological specimen of 32 patients with sebaceous adenocarcinomas of the eyelid. The expression of E-cadherin, β-catenin, and Ki-67 nuclear antigen was analyzed by immunohistochemistry. RESULTS: The membranous expression of E-cadherin and of β-catenin was reduced in 17 (53%) patients and 18 (56%) patients, respectively, while elevated cytoplasmic levels and a nuclear accumulation of β-catenin were found in 27 (84%) and six (19%) patients, respectively. The low expression of membranous β-catenin was associated with a high degree of tumor infiltration (p = 0.02), high amount of inflammation (p = 0.01), and high degree of poor tumor differentiation (p < 0.001). In a similar manner, a low membranous E-cadherin expression in tumors was significantly associated with poor tumor differentiation (p = 0.001) and high tumor cell proliferation rate (p = 0.004). The Ki-67 proliferation index was significantly higher in the group with low membranous E-cadherin expression than in the group with high membranous E-cadherin expression (62.3 ± 20.8 versus 42.1 ± 15.3; p = 0.004). The membranous E-cadherin expression and the membranous β-catenin expression were significantly associated with each other (Fisher's exact probability test, p = 0.03). CONCLUSIONS: Sebaceous eyelid adenocarcinomas showed a decrease in the membranous expression of E-cadherin and of β-catenin. These changes were associated with poor tumor differentiation and an increase in the tumor infiltration and inflammation, pointing at a potential role of a low E-cadherin and low β-catenin expression for poor prognosis of sebaceous eyelid adenocarcinomas. Correspondingly, the cell proliferation index and the expression of E-cadherin were inversely correlated with each other. The findings suggest that the immunohistochemical detection of a low E-cadherin and low β-catenin expression may help in the examination and staging of sebaceous eyelid adenocarcinomas.
BACKGROUND: Since the E-cadherin-catenin complex is of importance in the cancerogenesis of epithelial tumors, we examined the expression of E-cadherin and β-catenin in sebaceous adenocarcinomas of the eyelid and correlated the results with clinicopathological parameters. METHODS: The study included histological specimen of 32 patients with sebaceous adenocarcinomas of the eyelid. The expression of E-cadherin, β-catenin, and Ki-67 nuclear antigen was analyzed by immunohistochemistry. RESULTS: The membranous expression of E-cadherin and of β-catenin was reduced in 17 (53%) patients and 18 (56%) patients, respectively, while elevated cytoplasmic levels and a nuclear accumulation of β-catenin were found in 27 (84%) and six (19%) patients, respectively. The low expression of membranous β-catenin was associated with a high degree of tumor infiltration (p = 0.02), high amount of inflammation (p = 0.01), and high degree of poor tumor differentiation (p < 0.001). In a similar manner, a low membranous E-cadherin expression in tumors was significantly associated with poor tumor differentiation (p = 0.001) and high tumor cell proliferation rate (p = 0.004). The Ki-67 proliferation index was significantly higher in the group with low membranous E-cadherin expression than in the group with high membranous E-cadherin expression (62.3 ± 20.8 versus 42.1 ± 15.3; p = 0.004). The membranous E-cadherin expression and the membranous β-catenin expression were significantly associated with each other (Fisher's exact probability test, p = 0.03). CONCLUSIONS:Sebaceous eyelid adenocarcinomas showed a decrease in the membranous expression of E-cadherin and of β-catenin. These changes were associated with poor tumor differentiation and an increase in the tumor infiltration and inflammation, pointing at a potential role of a low E-cadherin and low β-catenin expression for poor prognosis of sebaceous eyelid adenocarcinomas. Correspondingly, the cell proliferation index and the expression of E-cadherin were inversely correlated with each other. The findings suggest that the immunohistochemical detection of a low E-cadherin and low β-catenin expression may help in the examination and staging of sebaceous eyelid adenocarcinomas.
Authors: Sam M Wiseman; Hamid Masoudi; Paddy Niblock; Dmitry Turbin; Ashish Rajput; John Hay; Douglas Filipenko; David Huntsman; Blake Gilks Journal: Am J Surg Date: 2006-05 Impact factor: 2.565
Authors: Tjinta Brinkhuizen; Karin van den Hurk; Véronique J L Winnepenninckx; Joep P de Hoon; Ariënne M van Marion; Jürgen Veeck; Manon van Engeland; Maurice A M van Steensel Journal: PLoS One Date: 2012-12-17 Impact factor: 3.240