INTRODUCTION: Following yellow fever virus (YFV) isolation in monkeys from the São José do Rio Preto region and two fatal human autochthonous cases from the Ribeirão Preto region, State of São Paulo, Brazil, two expeditions for entomological research and eco-epidemiological evaluation were conducted. METHODS: A total of 577 samples from humans, 108 from monkeys and 3,049 mosquitoes were analyzed by one or more methods: virus isolation, ELISA-IgM, RT-PCR, histopathology and immunohistochemical. RESULTS: Of the 577 human samples, 531 were tested by ELISA-IgM, with 3 positives, and 235 were inoculated into mice and 199 in cell culture, resulting in one virus isolation. One sample was positive by histopathology and immunohistochemical. Using RT-PCR, 25 samples were processed with 4 positive reactions. A total of 108 specimens of monkeys were examined, 108 were inoculated into mice and 45 in cell culture. Four virus strains were isolated from Alouatta caraya. A total of 931 mosquitoes were captured in Sao Jose do Rio Preto and 2,118 in Ribeirão Preto and separated into batches. A single isolation of YFV was derived from a batch of 9 mosquitoes Psorophora ferox, collected in Urupês, Ribeirão Preto region. A serological survey was conducted with 128 samples from the municipalities of São Carlos, Rincão and Ribeirão Preto and 10 samples from contacts of patients from Ribeirão Preto. All samples were negative by ELISA-IgM for YFV. CONCLUSIONS: The results confirm the circulation of yellow fever, even though sporadic, in the Sao Paulo State and reinforce the importance of vaccination against yellow fever in areas considered at risk.
INTRODUCTION: Following yellow fever virus (YFV) isolation in monkeys from the São José do Rio Preto region and two fatal human autochthonous cases from the Ribeirão Preto region, State of São Paulo, Brazil, two expeditions for entomological research and eco-epidemiological evaluation were conducted. METHODS: A total of 577 samples from humans, 108 from monkeys and 3,049 mosquitoes were analyzed by one or more methods: virus isolation, ELISA-IgM, RT-PCR, histopathology and immunohistochemical. RESULTS: Of the 577 human samples, 531 were tested by ELISA-IgM, with 3 positives, and 235 were inoculated into mice and 199 in cell culture, resulting in one virus isolation. One sample was positive by histopathology and immunohistochemical. Using RT-PCR, 25 samples were processed with 4 positive reactions. A total of 108 specimens of monkeys were examined, 108 were inoculated into mice and 45 in cell culture. Four virus strains were isolated from Alouatta caraya. A total of 931 mosquitoes were captured in Sao Jose do Rio Preto and 2,118 in Ribeirão Preto and separated into batches. A single isolation of YFV was derived from a batch of 9 mosquitoes Psorophora ferox, collected in Urupês, Ribeirão Preto region. A serological survey was conducted with 128 samples from the municipalities of São Carlos, Rincão and Ribeirão Preto and 10 samples from contacts of patients from Ribeirão Preto. All samples were negative by ELISA-IgM for YFV. CONCLUSIONS: The results confirm the circulation of yellow fever, even though sporadic, in the Sao Paulo State and reinforce the importance of vaccination against yellow fever in areas considered at risk.
Authors: Marco A B de Almeida; Edmilson Dos Santos; Jáder da C Cardoso; Lucas G da Silva; Rafael M Rabelo; Júlio César Bicca-Marques Journal: Ecohealth Date: 2018-12-17 Impact factor: 3.184
Authors: Ana Freitas Ribeiro; Ciléa Tengan; Helena Keico Sato; Roberta Spinola; Melissa Mascheretti; Ana Cecilia Costa França; Marcio Port-Carvalho; Mariza Pereira; Renato Pereira de Souza; Marcos Amaku; Marcelo Nascimento Burattini; Francisco Antonio Bezerra Coutinho; Luis Fernandez Lopez; Eduardo Massad Journal: Mem Inst Oswaldo Cruz Date: 2015-02-27 Impact factor: 2.743
Authors: Andrezza C Chagas; Eric Calvo; Claudia M Rios-Velásquez; Felipe A C Pessoa; Jansen F Medeiros; José M C Ribeiro Journal: BMC Genomics Date: 2013-12-13 Impact factor: 3.969
Authors: Eduardo S Moreno; Ilaria Agostini; Ingrid Holzmann; Mario S Di Bitetti; Luciana I Oklander; Martín M Kowalewski; Pablo M Beldomenico; Silvina Goenaga; Mariela Martínez; Eduardo Lestani; Arnaud L J Desbiez; Philip Miller Journal: Mem Inst Oswaldo Cruz Date: 2015-10-23 Impact factor: 2.743