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Literature DB >> 21731574

Solubility Limits of Cholesterol, Lanosterol, Ergosterol, Stigmasterol, and β-Sitosterol in Electroformed Lipid Vesicles.

Mark M Stevens¹, Aurelia R Honerkamp-Smith, Sarah L Keller.

Abstract

Here we use nuclear magnetic resonance to measure the solubility limit of several biologically relevant sterols in electroformed giant unilamellar vesicle membranes containing phosphatidylcholine (PC) lipids in ratios of 1:1:X DOPC:DPPC:sterol. We find solubility limits of cholesterol, lanosterol, ergosterol, stigmasterol, and β-sitosterol to be 65-70%, ~35%, 30-35%, 20-25%, and ~40%, respectively. The low solubilities of stigmasterol and β-sitosterol, which differ from cholesterol only in their alkyl tails, show that subtle differences in tail structure can strongly affect sterol solubility. Below the solubility limits, the fraction of sterol to PC-lipid in electroformed vesicles linearly reflects the fraction in the original stock solutions used in the electroformation process.

Entities: CellLine Chemical Disease Gene Species

Year: 2010 PMID： 21731574 PMCID： PMC3124637 DOI： 10.1039/c0sm00373e

Source DB: PubMed Journal: Soft Matter ISSN： 1744-683X Impact factor: 3.679

40 in total

1. Critical fluctuations in domain-forming lipid mixtures.

Authors: Sarah L Veatch; Olivier Soubias; Sarah L Keller; Klaus Gawrisch
Journal: Proc Natl Acad Sci U S A Date: 2007-10-25 Impact factor: 11.205

2. Effect of sterol structure on the bending rigidity of lipid membranes: A (2)H NMR transverse relaxation study.

Authors: Greger Orädd; Vahid Shahedi; Göran Lindblom
Journal: Biochim Biophys Acta Date: 2009-06-30

3. Phase diagram of stigmasterol-dipalmitoylphosphatidylcholine mixtures dispersed in excess water.

Authors: Ruiguang Wu; Lin Chen; Zhiwu Yu; Peter J Quinn
Journal: Biochim Biophys Acta Date: 2006-05-05

4. Quantitative characterization of coexisting phases in DOPC/DPPC/cholesterol mixtures: comparing confocal fluorescence microscopy and deuterium nuclear magnetic resonance.

Authors: Janos Juhasz; Frances J Sharom; James H Davis
Journal: Biochim Biophys Acta Date: 2009-10-23

5. A calorimetric and spectroscopic comparison of the effects of ergosterol and cholesterol on the thermotropic phase behavior and organization of dipalmitoylphosphatidylcholine bilayer membranes.

Authors: David A Mannock; Ruthven N A H Lewis; Ronald N McElhaney
Journal: Biochim Biophys Acta Date: 2009-09-15

6. Lipid domains in giant unilamellar vesicles and their correspondence with equilibrium thermodynamic phases: a quantitative fluorescence microscopy imaging approach.

Authors: M Fidorra; A Garcia; J H Ipsen; S Härtel; L A Bagatolli
Journal: Biochim Biophys Acta Date: 2009-08-21

7. Properties of mixtures of cholesterol with phosphatidylcholine or with phosphatidylserine studied by (13)C magic angle spinning nuclear magnetic resonance.

Authors: Richard M Epand; Alex D Bain; Brian G Sayer; Diana Bach; Ellen Wachtel
Journal: Biophys J Date: 2002-10 Impact factor: 4.033

8. Evidence for regular distribution of sterols in liquid crystalline phosphatidylcholine bilayers.

Authors: P L Chong
Journal: Proc Natl Acad Sci U S A Date: 1994-10-11 Impact factor: 11.205

9. Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol.

Authors: Sarah L Veatch; Sarah L Keller
Journal: Biophys J Date: 2003-11 Impact factor: 4.033

10. Lipid dynamics and domain formation in model membranes composed of ternary mixtures of unsaturated and saturated phosphatidylcholines and cholesterol.

Authors: Dag Scherfeld; Nicoletta Kahya; Petra Schwille
Journal: Biophys J Date: 2003-12 Impact factor: 4.033

14 in total

10. Confocal Microscopy Confirmed that in Phosphatidylcholine Giant Unilamellar Vesicles with very High Cholesterol Content Pure Cholesterol Bilayer Domains Form.

Authors: Marija Raguz; Suresh N Kumar; Mariusz Zareba; Nada Ilic; Laxman Mainali; Witold K Subczynski
Journal: Cell Biochem Biophys Date: 2019-10-17 Impact factor: 2.194

Solubility Limits of Cholesterol, Lanosterol, Ergosterol, Stigmasterol, and β-Sitosterol in Electroformed Lipid Vesicles.

1. Critical fluctuations in domain-forming lipid mixtures.

2. Effect of sterol structure on the bending rigidity of lipid membranes: A (2)H NMR transverse relaxation study.

3. Phase diagram of stigmasterol-dipalmitoylphosphatidylcholine mixtures dispersed in excess water.

4. Quantitative characterization of coexisting phases in DOPC/DPPC/cholesterol mixtures: comparing confocal fluorescence microscopy and deuterium nuclear magnetic resonance.

5. A calorimetric and spectroscopic comparison of the effects of ergosterol and cholesterol on the thermotropic phase behavior and organization of dipalmitoylphosphatidylcholine bilayer membranes.

6. Lipid domains in giant unilamellar vesicles and their correspondence with equilibrium thermodynamic phases: a quantitative fluorescence microscopy imaging approach.

7. Properties of mixtures of cholesterol with phosphatidylcholine or with phosphatidylserine studied by (13)C magic angle spinning nuclear magnetic resonance.

8. Evidence for regular distribution of sterols in liquid crystalline phosphatidylcholine bilayers.

9. Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol.

10. Lipid dynamics and domain formation in model membranes composed of ternary mixtures of unsaturated and saturated phosphatidylcholines and cholesterol.

1. Comparative Study of the Condensing Effects of Ergosterol and Cholesterol.

2. Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation.

3. Effect of Electrical Parameters and Cholesterol Concentration on Giant Unilamellar Vesicles Electroformation.

4. Minimal effect of lipid charge on membrane miscibility phase behavior in three ternary systems.

5. Differential effect of plant lipids on membrane organization: specificities of phytosphingolipids and phytosterols.

6. Chemical Exchange Saturation Transfer (CEST) Signal at -1.6 ppm and Its Application for Imaging a C6 Glioma Model.

7. Depletion with Cyclodextrin Reveals Two Populations of Cholesterol in Model Lipid Membranes.

8. cDICE method produces giant lipid vesicles under physiological conditions of charged lipids and ionic solutions.

9. Vesicle Leakage Reflects the Target Selectivity of Antimicrobial Lipopeptides from Bacillus subtilis.

10. Confocal Microscopy Confirmed that in Phosphatidylcholine Giant Unilamellar Vesicles with very High Cholesterol Content Pure Cholesterol Bilayer Domains Form.